Evaluation of P53, Hox D10 and E Cadherin Status in Breast Cancer and Correlation with Histological Grade.

Preethi, S (2013) Evaluation of P53, Hox D10 and E Cadherin Status in Breast Cancer and Correlation with Histological Grade. Masters thesis, Madras Medical College, Chennai.

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Abstract

Breast Carcinoma Is The Most Common Cause Of Cancer Related Mortality In Urban Indian Women Overtaking Cervical Cancer. The Incidence Is 30 – 33 Per 1,00,000 Women In Urban India And It Is The Second Commonest Cause In Rural Women.1There Is A Gradual Rise In The Incidence Of Breast Carcinoma Worldwide. It Accounts For About 23% Of All Cancers In Women.2 Early Detection And Treatment Can Certainly Reduce The Mortality Rates. Breast Cancers Exhibit Widely Varying Behaviour With Regard To The Likelihood Of Recurrence, Metastasis And Response To Therapy.Study Of Tumour Molecular Characteristics Has Enhanced Our Understanding Of Both The Risk Of Breast Cancer Recurrence And The Response To Therapy. Hundreds Of Putative Markers Have Been Identified By Immunohistochemistry Or Bioassays And Thus Serve As An Important Prognostic And Predictive Factor. S O Me Of The Genes Implicated In Breast Cancer Progression And Evaluated In This Study Include The P53, E Cadherin And The Hoxd10 Gene. One Of The Most Commonly Mutated Genes In Human Cancer Is P53.3Alteration Of This Tumour Suppressor Gene Is A Critical Step In The Development Of Cancers.4 The Gene Is Present On Chromosome 17p, And Produces A Nuclear Phosphoprotein. The Protein Functions As A Transcription Factor And Regulates Entry Into S Phase Of The Cell Cycle.5,6 The P53 Protein Also Influences The Occurrence Of Apoptosis In Tumour Cells.7 Research Has Shown P53 Gene Mutation In Breast Cancers Is Associated With Worse Prognosis.8IHC Detects Mutant P53 Protein In The Cells As A Result Of Conformational Changes In The Polypeptide Which Results In Increased Stability.9 E Cadherin (EC) Is A Calcium Regulated Adhesion Molecule Present In Most Normal Epithelial Cells And Is A Classic Tumour Suppressor Gene.10 The EC Gene, CDH1 Is Located On Chromosome 16q22.1.2.11It Helps In Formation Of Glands, Epithelial Polarization And Stratification.12Loss Of EC Results In Dedifferentiation And Invasion In Carcinomas.13 While Majority Of The Infiltrating Lobular Carcinomas Show A Complete Loss Of EC Expression, Ductal Carcinomas Show Heterogeneous Loss Of EC Expression, Due To Epigenetic Transcriptional Downregulation.14 Studies Show CDH1 Under Expression To Be Associated With Histological Type, Higher Tumour Grade, Stage And Nodal Status.15 The Hox Gene Network Is Essential For Spatio Temporal Cell Localisation And For Cell To Cell Signal Decoding So As To Attain Phenotype Cell Identity.16 The Thoracic Hox Genes Are Involved In Breast Organogenesis,17 Whereas The Cervical And Lumbo-Sacral Hox Genes Are Involved In Progression Of Breast Cancer.18 The Genes Indicated In Breast Cancer Progression Include Hoxd10, B13, A11 In Lumbo Sacral Part And Hoxb2, D3, D4 In Cervical Part.19 In This Study Of 60 Cases Of Invasive Ductal Carcinoma NOS, An Attempt Is Made To Evaluate The P53 Status By IHC, The Hoxd10 And E Cadherin Status By PCR And To Correlate Them With Histological Grade And Other Prognostic Factors.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Breast Cancer ; P53, Hox D10 ; E Cadherin Status ; Correlation ; Histological Grade.
Subjects: MEDICAL > Pathology
Depositing User: Subramani R
Date Deposited: 23 Jun 2017 11:45
Last Modified: 23 Jun 2017 11:45
URI: http://repository-tnmgrmu.ac.in/id/eprint/355

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