Vasuki, K (2014) Pharmacognostical, Phytochemical Studies and Evaluation of Antidiabetic Potential of Biophytum sensitivum DC. and Biophytum intermedium Wt. Doctoral thesis, (Synopsis) The Tamilnadu Dr. M.G.R. Medical University, Chennai.
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Abstract
INTRODUCTION : The most ancient and celebrated treatises on Hindu medicine are no doubt the Ayurveda. India also possesses a great heritage of other ancient systems of medicine such as Siddha, Unani and Homeopathy. In addition to these traditional systems, there also exists in India a vast knowledge of tribal and folk medicine, which utilize around 7,500 species of plants as medicine. Some of the ethno botanically important species have also provided leads for production of modern drugs by pharmaceutical companies. It is estimated that in India 90% of the prescriptions contain plant products. Ayurvedic and other traditional systems of Indian medicines fully depend on wild plants for preparation of drugs. AIM AND OBJECTIVES: Aim: To scientifically validate the selected rare medicinal plants Biophytum sensitivum DC. and Biophytum intermedium Wight in terms of Pharmacognostical, Phytochemical and Pharmacological investigations with special reference to anti-diabetic property. OBJECTIVES: 1. To fix the pharmacognostical standards for the selected rare medicinal plants with special reference to the macroscopical and microscopical evaluation including powder microscopy, quantitative microscopy and proximate analysis. 2. To isolate and characterize the phyto constituents responsible for exhibiting anti diabetic property. 3. To give scientific evidence for the treatment of anti diabetic property. 4. To show the potential of different diet pattern of people with normal food and high fat diet. MATERIALS AND METHODS: MATERIALS: The selected medicinal plants, Biophytum sensitivum DC. and Biophytum intermedium Wight are distributed in Tuticorin and Kuttralam respectively and they were freshly collected in the month of December and November 2012 respectively. They were authenticated (Specimen no. PARC 2013/2013 and PARC 2013/2012 respectively ) by botanist Dr. P. Jayaraman, Director, Plant Anatomy Research Centre (PARC), Chennai, Tamilnadu, India. The whole parts of both plants were taken for the present study. METHODS: BSC 1 and BIC 2 were analyzed using UHPLC + Focused with reverse-phase Acclaim 120, RP-C18 120 Å, 2.1 × 150mm, 3.0μm column (Dionex, USA). Mass analyzer Quadrupole II and Detector TOF (Bruker, Germany). Experimental conditions used for LC-MS/MS analysis were given below. LC conditions: LC-MS analysis was set with UV at 325nm, at the flow rate of 0.2mL/min, gradient mobile system start with 0.2 min at 99% CAN (1% acetic acid) and 1% water to 75% water at 16th minute, this was brought to 99% water in 19th minute and was maintained at same condition until run ends for another at 23rd minute. MS conditions: MS analysis was performed using ESI in the negative mode, Nebulizer pressure was 30.5psi with 6.0 l/min N2 flow, m/z range: 50-1000m/z, Capillary voltage was 4500V, dry heat temperature at 280oC. Mass spectroscopy provides molecular weight for the isolated compounds. It relies of production of ions from a parent compound and the subsequent characterization of the pattern that are produced. RESULTS : In pharmacognostical studies, the macroscopical, microscopical studies of leaflet, stem and root were performed to ascertain the arrangement of tissues of both selected medicinal plants were evaluated. The T.S. of leaflet through midrib, crystal distribution around the vascular bundle of the midrib, leaf lamina, leaf margin, stomata, T.S. of stem, T.S. of root and the powder analysis of both plants were evaluated. Quantitative microscopy, physico-chemical evaluation, extractive values and fluorescence analysis for both plants were determined. CONCLUSION : Diabetes mellitus is the most common endocrine disorder that affects more than 285 million people worldwide. The number is expected to grow to 438 million by 2030, corresponding to 7.8% of the adult population. In addition to the primary effects of diabetes, this disease is accompanied by increased risk factors such as hyperglycemia, hypertension, dyslipidemia, severe atherosclerosis etc. Though there are wide developments in the treatment of diabetes mellitus by synthetic drugs, side effects such as hypoglycemia at higher dose administration, low oral bioavailability due to degradation in stomach makes it necessary to find other alternatives. Therefore, there is an owing interest in discovering natural treatments without negative side effects that can reduce these risk factors in diabetic patients. In conclusion, ethanolic extracts of whole plants of Biophytum sensitivum DC. And Biophytum intermedium Wight showed significant antidiabetic and antioxidant effect in diabetic rats after oral administration. It could be speculated that the observed antihyperglycemic and antioxidant activity of Biophytum sensitivum DC. and Biophytum intermedium Wight due to the presence of flavonoid glycosides as active constituents. The present investigation has also opened an avenue for further research especially with reference to the development of potent formulation for diabetes mellitus from the whole plants of Biophytum sensitivum DC. and Biophytum intermedium Wight.
| Item Type: | Thesis (Doctoral) |
|---|---|
| Uncontrolled Keywords: | synopsis, Pharmacognostical, Phytochemical studies, Evaluation, Antidiabetic Potential, Biophytum sensitivum DC. , Biophytum intermedium Wight. |
| Subjects: | PHARMACY > Pharmaceutics |
| Depositing User: | Subramani R |
| Date Deposited: | 20 Aug 2017 13:31 |
| Last Modified: | 24 Oct 2022 14:19 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/2749 |
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