Latha, S T (2012) Identification and isolation of bio-active constituents from aerial parts of gomphrena globosa for cancer therapy. Doctoral thesis, (Synopsis) The Tamilnadu Dr. M.G.R. Medical University, Chennai.
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Abstract
INTRODUCTION : Cancer is a group of diseases characterized by uncontrolled division of cells in the part of the body and is able to invade to other tissues or parts of the body through blood and lymph system. Cancer is of different types that based on the organ or type of cell in which they start. Different cancer types like cancer of lung, esophagus, stomach, oral, pharynx, colon and skin, are affecting the human population. In the developed world, cancer is the leading cause of death whereas in the developing world it is the second leading cause of death as evidenced by the projected number of new cases and death worldwide. About 12.7 million people were suffering from cancer and 7.6 million people died of cancer in 2008 worldwide. Of the above estimate 56% of the cancer cases and 64% of the deaths were reported in the developing countries. Cancer burden in India is lower than that of developed countries like United States, and WHO, projected the dramatic rise in cancer burden to 20 million by 2020 with 70% in the developing countries. The cause for higher cancer burden in developing countries is due to population growth and ageing that would have massive impact on the number of new cancer cases and deaths. In India, the more prevalent cancers in men are lung, esophagus, stomach, oral and pharynx, while in women, breast and cervix cancers followed by stomach and esophagus. Recently it has been reported that there were more than 5 lakes people died of cancer in India in the year 2010, and in female most deaths were due to breast cancer. AIM AND OBJECTIVES : The present study aimed to investigate the plants Celosia cristata and Gomphrena globosa, for anticancer property by invitro method and select the best plant with potent antitumor property and investigate the plant in detail. The objectives of the study are as follows: Authentication of the selected plant. • Successive extraction of the plant material using non-polar to polar solvents. • Screening of the plant extracts for anticancer property by in-vitro. • Selection of the plant extract with potential anticancer property. • Toxicity study on the selected plant extract. • Screening of the selected plant extract for anticancer property by in-vivo. • Phytochemical screening of the selected plant extract. • Isolation and structural elucidation of the active constituent(s) from the extract. • Screening of the active constituent(s) for anticancer property by in-vitro and in-vivo. Studying the mechanism of anticancer activity of the active constituent. MATERIALS AND METHODS : Materials: The selected plants, Celosia cristata and Gomphrena globosa are widely distributed in and around Tiruchengode of Namakkal district. They were freshly collected in the month of June and authenticated (Specimen No. 1367, 1368) by Dr. G. V. S. Murthy, Joint Director, Botanical Survey of India, Coimbatore. The aerial parts of both the plants were taken for the present study. Extraction : Aerial parts of Celosia cristata and Gomphrena globosa were selected for preliminary screening of anticancer property by in-vitro. Both the plants were dried separately under shade and then powdered to coarse. Powdered material (500 g) of both the plants was separately extracted with chloroform, ethanol and water by continuous hot extraction method for 48 h by using Soxhlet apparatus 40. The extracts were concentrated to a dry mass by vacuum distillation. After complete drying the extracted materials were weighed and their extractive value in percentage was calculated with reference to the air dried sample. All these extracts were subjected to in-vitro anticancer study by Trypan blue method. RESULTS : Preliminary screening of in-vitro cytotoxic study of extracts From the results it was observed that the chloroform extract of Gomphrena globosa , produced cytotoxic activity with inhibitory concentration (IC50) of 45.94 Pg/ml for DLA and 56.87 Pg/ml for ECA. And the effect was relatively more potent than other extracts of the same plant or of Celosia cristata and therefore the chloroform extract of Gomphrena globosa was selected for further studies. CONCLUSION : * Two plants, Celosia cristata and Gomphrena globosa, were initially studied for anticancer activity by in-vitro method against DLA and ECA cell lines. * The chloroform extract of Gomphrena globosa was found to be potential cytotoxic activity. * The CEGG was further screened in detail for anticancer activity against invitro human cell lines and in-vivo DLA and ECA cell lines. * The compound possessing potent anticancer activity against human cell lines MCF – 7, DU 145, Hela and A-431 was isolated and structurally elucidated by TLC, IR, 1H NMR, 13C NMR and LC-MS spectral data. * The isolated compound was found to be Oleuropein. * The compound Oleuropein was found to possess anticancer activity through antioxidant, antiradical and cell apoptosis mechanism. In conclusion, the results of the study demonstrated that the plant, Gomphrena globosa possess anticancer activity and the compound responsible for this activity was found to be Oleuropein. Further studies are recommended in humans for effective use of this compound in cancer.
| Item Type: | Thesis (Doctoral) |
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| Uncontrolled Keywords: | synopsis, Identification, isolation, bio-active constituents, aerial parts, gomphrena globosa, cancer therapy. |
| Subjects: | PHARMACY > Pharmaceutics |
| Depositing User: | Subramani R |
| Date Deposited: | 20 Aug 2017 13:02 |
| Last Modified: | 24 Oct 2022 14:13 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/2746 |
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