Production, Characterization and Uses of a Fungal Biopolymer Isolated from Different Sources: A Comparison

Bharathi, P (2008) Production, Characterization and Uses of a Fungal Biopolymer Isolated from Different Sources: A Comparison. Masters thesis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore.

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Abstract

Chitin is highly insoluble N - acetylated polymer of β- (1, 4)-D-glucosamine. Chitosan is an acid soluble deacetylated form of chitin. Chitin is commonly found in exoskeleton of marine invertebrates and cuticles of insects. Both chitin and chitosan are also present in the cell wall of most fungi. Chitosan can be obtained by deacetylating chitin prepared from crab or shrimp shell. However, this process fails to produce chitosan of uniform quality. The physicochemical characteristics of chitosan depend on the degree of deacetylation and it also differs, based on the crustacean species and preparation methods used. Chitosan can also be obtained from filamentous fungi. No chemical deacetylation is necessary in this process. As a result, the quality of fungal chitosan is more consistent, inexpensive, eco friendly and chitin and chitosan are extracted simultaneously. Therefore the physicochemical properties and yield of chitosan isolated directly from fungi may be optimized by controlled fermentation. In this work fungal chitosan was prepared from Aspergillus niger, Rhizopus oryzae and Mucor hiemalis without using strong alkaline solution normally used for deacetylation. Fungal chitosan was isolated, with 85-95% degree of deacetylation and viscosity of 13-18 (cP). The profile of both isolated and commercial chitosan showed similar FT-IR and 1HNMR spectra. The antioxidant activity of the isolated chitosan was studied using the stable DPPH (2, 2 diphenyl- picryl- hydrazylhydrate) free radical method. Ascorbic acid was used as standard. The fungal chitosan showed maximum scavenging of DPPH free radicals (EC 50 value was in the range of 3.1- 5.3). The results suggest that chitosan possesses significant in vitro antioxidant activity compared with ascorbic acid. The antibacterial activity of the isolated chitosan was studied by Kirby-Bauer method and it showed good activity against S. aureus, E.coli, including clinical isolate of E.coli.

Item Type: Thesis (Masters)
Additional Information: Reg No. 26063652
Uncontrolled Keywords: Production ; Characterization ; Fungal Biopolymer Isolated ; Different Sources ; Comparison.
Subjects: PHARMACY > Pharmaceutical Biotechnology
Depositing User: Subramani R
Date Deposited: 20 Aug 2017 03:18
Last Modified: 15 May 2018 15:50
URI: http://repository-tnmgrmu.ac.in/id/eprint/2721

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