Venkatesh Karthikeyan, T (2007) Method Development and Validation for Simultaneous Estimation of Aceclofenac, Paracetamol & Chlorzoxazone in Tablets by HPLC, HPTLC & Aceclofenac By Differential Spectroscopy. Masters thesis, College of Pharmacy, Madras Medical College, Chennai.
|
Text
2602184venkateshkarthikeyant.pdf Download (653kB) | Preview |
Abstract
This studies number of drugs and drug formulations introduced into the market is increasing at an alarming rate. The complexity of these dosage forms poses considerable challenge to the analytical chemist during the development of assay procedure. The estimation of individual drugs and multi component dosage forms become difficult due to cumbersome extraction and isolation procedure. For the estimation of the drugs present in formulations, methods like HPLC, HPTLC and spectrophotometric methods are more suitable since these methods are highly specific, linear, precise, accurate, sensitive and rapid. From the review of literature, we found no documentary evidence is available for the quantitative analysis of ternary mixture, Aceclofenac, paracetamol and chlorzoxazone. We also found that there is no reported method for the estimation of drugs by differential spectrometry. In the proposed work, we developed HPLC and HPTLC method to quantify aceclofenac in combination with paracetamol and chlorzoxazone and individually using differential spectroscopy. The formulation containing aceclofenac 100mg, paracetamol 500 mg and chlorzoxazone 500 mg were estimated by RP-HPLC and HPTLC methods. The chromatographic conditions like detection wavelength, the composition of mobile phase, the pH of the mobile phase were optimized for the best possible separation of these drugs in the formulation. The drug formulation is then estimated using the optimized chromatographic conditions and the comparative datas were given in the table. Aceclofenac was estimated by differential spectropphotometric method. The results obtained by these methods including recovery studies were comparable which proves the repeatability and suitability of the methods.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Aceclofenac; Paracetamol; Chlorzoxazone; HPLC; HPTLC; chromatographic |
| Subjects: | PHARMACY > Pharmaceutical Chemistry |
| Depositing User: | Ravindran C |
| Date Deposited: | 17 Aug 2017 07:44 |
| Last Modified: | 17 Aug 2017 07:44 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/2669 |
Actions (login required)
 |
View Item |
