Parimaleshwari, R (2012) Simultaneous Estimation of Ondansetron HCL and Promethazine HCL by UV-Spectroscopy and Ondansetron HCL and Paracetamol by HPTLC in Bulk and Pharmaceutical Dosage Forms. Masters thesis, R V S College of Pharmaceutical Sciences, Coimbatore.
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Abstract
The Present studies is Literature survey revealed that various analytical methods like UVSpectrophotometry, HPLC and HPTLC methods, have been reported for the estimation of Ondansetron Hcl, Promethazine Hcl and Paracetamol, individually and combination with some other drugs. No methods for estimation of Ondansetron Hcl with Promethazine by using UV-Spectroscopy and Ondansetron Hcl with Paracetamol by using HPTLC. The review of literature prompted us to develop a new validated UV Spectroscopic method for the estimation of Ondansetron Hcl with Promethazine Hcl and a HPTLC method for Ondansetron with Paracetamol in pharmaceutical dosage forms. Aim: To develop a UV-Spectroscopic method for the Simultaneous estimation of Ondansetron Hcl with Promethazine Hcl in formulation. To develop and validate a HPTLC method for Simultaneous estimation of Ondansetron Hcl and Paracetamol in formulation. Objective: To procure pure samples from Bulk drug industry, To find the linearity range of the drugs to be estimated with suitable solvents & mobile phase using UV and HPTLC, To prepare the formulation dilution inside the linearity range to evaluate the drug content and To validate the developed method according to FDA, USP & ICH guidelines. In order to develop a UV and HPTLC effective most of the effort should be spent in method development and optimization as this will improve the final method performance. A well developed should be easy to validate. A method should be developed with the goal to rapidly preclinical sample, formulation prototype and commercial samples. Review of the literature on Ondansetron Hcl and Promethazine Hcl & Ondansetron and Paracetamol strongly indicates that there is no method available for UV and HPTLC method for Simultaneous determination and validation of Ondansetron Hcl and Promethazine Hcl & Ondansetron Hcl and Paracetaml in bulk and pharmaceutical dosage forms,but individual and combination with other drugs methods are available. The analytical procedure described for assay represents a specific,linear,precise,accurate and system suitable for Simultaneous determination of Ondansetron Hcl and Promethazine Hcl by UV-Spectroscopy and Ondansetron Hcl and Paracetamol by HPTLC in bulk and pharmaceutical dosage form. By observing the validation parameters,accuracy,precision,expressed as % RSD,specificity,linearity (correlation –coefficient). The validation methods can be employed for routine analysis of bulk and formulation for assay. The formulations were successfully analyzed using the developed methods. The results obtained from the validation parameters met the ICH,FDA and USP requirements as well as obeys Beer’s law.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Ondansetron HCL; Promethazine HCL; UV-Spectroscopy; Paracetamol; HPTLC |
| Subjects: | PHARMACY > Pharmaceutical Analysis |
| Depositing User: | Ravindran C |
| Date Deposited: | 10 Aug 2017 06:27 |
| Last Modified: | 10 Aug 2017 06:27 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/2622 |
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