Suresh, P (2008) Estimation of Alfuzosin Hydrochloride by UV-Visible Spectrophotometry and RP-HPLC. Masters thesis, Adhiparasakthi College of Pharmacy, Melmaruvathur.
|
Text
2605076sureshp.pdf Download (4MB) | Preview |
Abstract
The present studies is drug analysis plays an important role in the development, manufacture and therapeutic use of drugs. Most of the pharmaceutical industries do the quantitative chemical analysis to ensure that the raw material used and the final product thus obtained meet certain specification and to determine how much of each components are present in the final product. Extensive literature survey reveals that Afuzosin HC1 is a official drug of BP 2003 which recommends non-aqueous titration for estimation of Alfuzosin Hcl in bulk drug only where the end point is determined by potentiometry. The titrimetric method has its own limitation is that; it is very difficult to measure small quantity of drug sample and less sensitive. Further, literature review revealed, HPLC and HPTLC methods have been reported in biological fluids, bulk drug and pharmaceutical dosage form, but UV-Spectrophotometric method was not reported. Hence, an attempt is made in this project to develop simple, cost effective, sensitive, precise and accurate spectrophotometric methods HPLC for Alfuzosin HC1. Plan of the developed experimental protocol: Selection, Collection and Identification of Alfuzosin HC1 for analysis, Development of simple, cost effective and accurate spectrophotometric methods, Development of a rapid and accurate RP-HPLC method using UV detection. Analysis of marketed formulations of Alfuzosin HC1, Validation of proposed methods, Statistical analysis of developed analytical methods. The proposed analytical methods are simple, economical, rapid, sensitive, reproducible and accurate for the estimation of Alfuzosin HC1. The method adopted for our studies are, UV spectroscopic method, Visible spectroscopic method and RP-HPLC method. The drug samples were analyzed by UV spectroscopy method using 0.1M NaOH as solvent at λmax 350 nm and the content of drug present in each formulation was found to be, Alfoo - 9.81 mg (98.10%), Alfusin - 9.96 mg (99.60%) , Xelflo - 10.00 mg (100.00%). The drug samples were also analysed by visible spectroscopy by using Ion-pair complex method. The λmax was found at 416 nm. The content of drug present in each formulation was found to be, Alfoo - 9.92 mg (99.20%), Alfusin - 10.00 mg (100.00%), Xelflo - 9.84 mg (98.40%). Simultaneously, a newer RP-HPLC method was developed for both bulk drug and formulations. The proposed method gives reliable assay results with short analysis time (3.0 mins) using the mobile phase methanol: phosphate buffer- (pH 3) at λmax 245 nm. The content of drug present in the formulation, Alfoo was found to be 9.92 mg (99.20 %). All the above methods do not suffer from any interference due to common excipients. Therefore it was found that the proposed methods could be successfully applied to estimate commercial pharmaceutical products containing Alfuzosin HC1. Thus, the above studies and findings will enable the quantification of the drug for future investigation in the field of analytical chemistry.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Alfuzosin; Hydrochloride; UV-Visible Spectrophotometry; RP-HPLC; Xelflo |
| Subjects: | PHARMACY > Pharmaceutical Analysis |
| Depositing User: | Ravindran C |
| Date Deposited: | 09 Aug 2017 07:23 |
| Last Modified: | 10 Aug 2017 01:43 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/2601 |
Actions (login required)
 |
View Item |
