Elakkiya, M (2016) Safety and Pharmacological Profile of Thirikadugu Dravagam. Masters thesis, National Institute of Siddha, Chennai.
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Abstract
The drug Thirikadugu dravagam was selected from the siddha literature “yaakobu vaithiya chinthamani” 700 to evaluate the safety and Pharmacological profile in animal models. The ingredients of the test drug was identified and authenticated by Botanist, The standard operative procedure were followed. Review of various information Relevant to the study Both Modern and Siddha Aspect supported the study. According to AYUSH protocol Physical characterization, like PH, Specific Gravity were evaluated. PH of Thirikadugu dravagam is 4..65, Even though it is acidic, Siddhars indicated this for internal use with equal amount of water, to Neutralise the acid. Specific Gravity is commonly used in pharmaceutical laboratory to determine the concentration of the solution. Specific gravity of Thirikadugu dravagam is 0.9992. Elemental analysis is also essential to ensure the Safety and efficacy of drug. Test drug shows heavy metals below the detected limit. GC-MS: Have been used for identification of large number of components present in natural and biological systems. Hence the drug is consider as safe drug. chemical analysis: chemical analysis of the drug Thirikadugu dravagam reveals that the presence Chloride. Calcium, alkaoloids. All these analytical studies shows, The drug is safe and shows Sufficient Molecules of therapeutic benefits for the safety and efficacy of the Drug Thirikadugu Dravagam. Toxicological studies: Before going to clinical study, It is essential to evaluate the toxicity studies. These studies are important to Globalise the siddha system of medicine. Based on these Acute, Sub-acute, and Sub-chronic studies were carried out using wistar albino rats. This study reveals that, no significant toxic effect of the drug Thirikadugu dravagam upto the higher dose level of 10ml/kg in acute oral toxicity, sub- acute and sub chronic toxicity studies. Therefore the drug Thirikadugu dravagam is safe on animal experimentation, and there is no toxicity of administration. Hence it can be classified under the category of drug with non-toxic. Pharmacological studies: Pharmacological study was carried out in animal models The experimental data showed that Thirikadugu dravagam has bronchodilator, Anti-histaminic Anti-inflammatory activity and the results are as follows, Bronchodilator activity: The contractile of tracheal chain in vitro has often been utilized for the study of contractile response of agonists as well as antagonist. spasmogen such as histamine produces dose dependent contraction of guinea pig tracheal chain preparation. In the present study, the trial drug Thirikadugu dravagam significantly inhibited the histamine- induced contractions of isolated guinea pig tracheal chain preparation, indicating Bronchodilator activity Anti-histaminic activity: The result of present study suggested that guinea pig exposed to histamine aerosol showed singns of progressive Dysponea leading to convulsions. The two doses of Thirikadugu dravagam prolonged the latent period of convulsion as compared to control following the exposure to histamine aerosol. Thus our findings suggest that Thirikadugu dravagam possess significant antihistaminic activity. Anti- inflammatory activity: Thirikadugu dravagam exhibited significant anti-inflammatory activity when compare to the control. AIM AND OBJECTIVES: AIM: To evaluate the Safety and Pharmacological Profile of the Test Drug “THIRIKADUGU DRAVAGAM" in Animal Models. OBJECTIVES: Review of various information relevant to the study from Siddha and modern literature. Preparation of the drug according to Siddha literature. 1. Analytical study of the prepared drug a) Organoleptic & physical characterization, b) Chemical analysis to evaluate acidic and basic radicals, c) TLC & HPTLC Finger print analysis, d) Elemental analysis(ICPOES), e) Gas chromotography (GS-MC). 2. Toxicity studies: a. Acute oral toxicity study OECD – 423 Guideline. b. 28 day Repeated oral toxicity study OECD – 407Guideline. c. 90 days chronic toxicity study OECD – 408 Guideline. 3. Pharmacological activities in animal models: a. Bronchodilator activity (Isolated tracheal chain preparation method). b. Anti-histaminic activity of Thirikadugu dravagam in vivo. c. Anti-inflammatory activity (Carageenan induced paw odema). RESULTS: In My research, studies like Organoleptic characters, physical characters, chemical analysis, phytochemical analysis, Elemental analysis, Gas chromatography, Toxicity and pharmacological studies have been carried to know the potency and efficacy of the drug Thirikadugu dravagam. Botanical aspect explains the active principle and medicinal uses of the plants. Gunapadam review brings the effectiveness of the drug in treating Bronchial ashtma. The pharmacological review explains about the methodology of Bronchodilator, Anti-histaminic activity and the drugs used. Analytical study of the drug is more essential to derive the efficacy, potency of the drug by analysing it by various studies. Following are the results of physicochemical, Phytochemical analysis, estimation of basic and acidic radicals, Elemental analysis, GSMC have been done and tabulated. Toxicological results of the drug and pharmacological activity of the drug were derived. Its result has been tabulated and interpretation was made below. Thus it is to give a complete justification to bring the effectiveness of the trial drug Thirikadugu dravagam. SUMMARY: The test drug Thirikadugu dravagam was selected from the siddha literature “Yaaakobu vaithiyachinthamani” for its Bronchodilator, Antihistaminic and Antiinflammatory activities. The test drug was prepared by the given procedure. All the ingredients were identified and authenticated by the experts. Review of literature in various categories was carried out. Siddha aspect, botanical aspect disclosed about the drug. The drug was subjected to analysis such as physicochemical, photochemical, chemical analysis, ICPOES, GC-MS which provided the key ingredients present in the drug thus it accounts the efficacy of the drug Toxicological study was made according to OECD guidelines comprising acute, subacute and sub chronic toxicity study. It screens the safety of the drug. Pharmacological study was done. It revealed of Thirikadugu dravagam in animal model, Bronchodilator, Anti-histaminic activity using Guinea pig and Antiinflammatory activities in wistar albino rats. Results and discussion gives the proper justifications to prove the potency of the drug. Conclusion gives a compiled form of the study and explains the synergistic effect of all the key ingredients and activities that supports the study. Thus the herbal formulation Thirikadugu dravagam is validated for its Safety and efficacy for treating Bronchial asthma and it would be a great drug of choice. CONCLUSION: From the literature evidence, physicochemical analysis, Chemical analysis, Toxicological evaluation and Pharmacological studies, the drug Thirikadugu Drvagam has Bronchodilator, Anti-histaminic and anti-inflammatory activity. It is concluded that the drug Thirikadugu Dravagam can be used in the management of Bronchial asthma and the related Respiratory disorders.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Safety : Pharmacological Profile ; Thirikadugu Dravagam. |
| Subjects: | AYUSH > Gunapadam |
| Depositing User: | Ravindran C |
| Date Deposited: | 02 Aug 2017 09:04 |
| Last Modified: | 29 Dec 2018 14:55 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/2462 |
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