Biosynthesisa and Cytotoxicity of Kojic Acid from Aspergillus Niger.

Hassan Saeed, Abdelwahid Seedahmed (2013) Biosynthesisa and Cytotoxicity of Kojic Acid from Aspergillus Niger. Masters thesis, Ultra College of Pharmacy, Madurai.

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Aspergillus species are highly aerobic and are found in almost all oxygen-rich environments, where they commonly grow as molds on the surface of a substrate, as a result of the high oxygen tension. “In recent studies, increased levels of Reactive oxygen Species (ROS) were shown to be correlated with increased levels of aflatoxins biosynthesis in Aspergillus parasiticus , Commonly fungi grow on carbon- rich substrates such as monosaccharides (such as glucose) and polysaccharides (such as amylase). Aspergillus species are common contaminants of starchy foods (such as bread and potatoes), and grow in or many plants and trees. The selected fungal strain for our study is Aspergillus niger MTCC 2208 and was procured from IMTECH, Chandigarh and maintained in CYA slant, the fermentation process (submerged) was performed by adding 5 ml of Aspergillus niger MTCC 2208 inoculum culture in sterile Modified Czapek- Dox medium and was incubated at 25 ± 2o C for 14 days. Submerged fermentation technique was adapted to biosynthesize Kojic acid from Aspergillus niger. The highest production of kojic acid was obtained at a concentration of 55g glucose and 5g of peptone as an organic a nitrogen source and the yield was 1144.7μg, from this study we concluded that the production of Kojic acid increased with the increase of carbon source such as glucose and decreased with the decrease of organic nitrogen source such as peptone, the Kojic acid which was obtained from the 49 runs were pooled together and utilized to evaluate its antimicrobial and cytotoxic activity.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Biosynthesisa ; Cytotoxicity ; Kojic Acid ; Aspergillus Niger.
Subjects: PHARMACY > Pharmaceutical Biotechnology
Depositing User: Ravindran C
Date Deposited: 28 Jul 2017 04:06
Last Modified: 12 Oct 2017 02:54

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