Hitler, Ningthoujam (2014) Estimation and Validation of Gefitinib by using Rp- High Performance Liquid Chromatography. Masters thesis, Cherraan`s College of Pharmacy, Coimbatore.
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Abstract
In the present work, RP-HPLC method has been developed for the quantitative estimation of Gefitinib in Pharmaceutical formulations. A rapid and sensitive RP-HPLC Method with UV detection (246 nm) for routine analysis of Gefitinib in Pharmaceutical formulation was developed using gradient pump (LC-10AT vp pump), The separations were achieved on a Agilents’s 5μ C18 (2) 100A, 250X4.6mm, column with UV detection at 246 nm. Chromatography was performed with mobile phase containing a mixture of 0.1% Triflouroacetic acid and Methanol (35:65 v/v) with flow rate 1 ml/min and retention time at 3.8 min. In the range of 5 -30μg/ml, the linearity of Gefitinib shows a correlation co-efficient of 0.999. The proposed method was validated by determining sensitivity, accuracy, precision, linearity, selectivity and system suitability parameters.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Estimation; Gefitinib; Liquid Chromatography |
| Subjects: | PHARMACY > Pharmaceutical Analysis |
| Depositing User: | Ravindran C |
| Date Deposited: | 27 Jul 2017 07:17 |
| Last Modified: | 06 Oct 2017 03:39 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/2246 |
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