Janani, S (2022) Scientific Validation of Siddha Herbomineral Formulation Naaga Chendooram for Anti-Cancer Activity, Apoptosis inducing Potential (Anti Tumour) in MCF-7 and MDA-MB 231 Cell Lines and Antioxidant Activity using In-Vitro Methods. Masters thesis, Government Siddha Medical College, Chennai.
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Abstract
The test drug Naaga chendhooram is selected from the text VEERAMAMUNIVAR VAAGADA THIRATU for the evaluation of Anti- Cancerous,Anti-Oxidant and Anti-Inflammatory activities. ❖ The review literature reveals that the Naagam and Tumeric have anti-tumour, anti-oxidant and anti-inflammatory activites and it also scientifically proves that reduces the adverse effects of cytotoxic drugs like nausea, vomiting. ❖ The main ingredient of test drug is zinc which acts in the way of nanoparticles which easily absorbed by the cell thereby increses the bioavailability of test drug. ❖ Cytotoxic drugs usually affects the renal and causes hyperurecemia but in our test drug has Acyranthus bidentata prevents the renal from hyperurecemia and prevents the GI mucosa. ❖ Aim of the dissertation is to study the Anti-cancer,Anti-oxidant and Anti inflammatory activities. The test drug was prepared properly by given procedure. All the ingredients were identified and authenticated by expert of department of Gunapadam. The preparation of trial drug was standardized primarily by physicochemical analysis of the drug shows it is a Pale Brownish, soft in nature due to the particle size .As per the siddha literature diseases are caused due to change in derangement of three vital humours, the vital humour kabha is deranged and accompanied with ushna causes yoni puttru. In physicochemical analysis, the water soluble ash value of the test drug is6.367 ± 2.779% and acid soluble ash value is 0±0%. The loss on drying of the drug is1.053 ± 0.1747% and total ash value is 91.6 ± 3.7360% .The biochemical analysis shows the presence of Carbonates and Sulfates and Mercury. These content are having important role in physiological functions of the body. Microbial limit test shows it is free from microbial contamination. The phytochemical analysis reveals the presence of phenols, tannins, and saponin. Saponins acts as an immunological adjuvant by increasing the immune response for cancer patients. Tannins having anti inflammatory and immune-modulatory, which control symptoms of breast cancer. In heavy metal analysis result shows the toxic heavy metals such as Pb, Ar, Cd are in below detection limit (BDL). It is evident that the safety of siddha medicine has been proved by the modern scientific way. The FTIR shows the presence of Alkane, Aromatics, carboxylic acid. SEM analysis of NAAGA CHENDHOORAM shows that the uniform distribution of particle present size is 5μm which increase the efficacy and bioavailability of test drug. So very minimal quantity of medicine is enough to treat the disease. ❖ Anti-cancerous activity study was carried out for NAAGA CHENDHOORAM by MCF- 7 (Human breast cancer) cell line was initially procured from National Centre for Cell Sciences (NCCS), Pune, India by Center for Research on Molecular and Applied Sciences, Triruvanandhapuram, Kerala, India. The cells maintained in vivo in Swiss albino mice by intraperitoneal transplantation. While transforming the tumor cells seeding in 96 well plate. The result shows that NAAGA CHENDHOORAM 100μg/ml shows 68.37 percentage viability. Tumour viable cell count and brought back the haematological parameter to more or less normal level. The result showed that there was a concentration dependant anti-oxidant activity of NAAGA CHENDHOORAM. At the concentration [12.5-200μgm/ml] Percentage of inhibition increased from 9.8 to 88%. At the concentration of 200 μgm/ml there was an increased percentage of inhibition (43.351%) is scavenging the free radicals (DPPH). It is showed that NAAGA CHENDHOORAMis having significant anti oxidant activity. Microbiology analysis result shows that NAC controls bacterial cause of Chronic ulcer which is one of the causative factor of cancer. The acute toxicity study shows that NAAGA CHENDHOORAM did not produce any toxic effect at dose of 5mg/kg, 50mg/kg, 300mg/kg, 1000mg/kg, and 2000mg/kg. In sub acute toxicity test drug NAAGA CHENDHOORAM is did not cause either any lethality or adverse changes with general behavior of rats and also there were no observable changes in hematological and biochemical parameter (50 to 200mg/kg body weight) over a period of 28 days. Our result have demonstrated that the NAAGA CHENDHOORAM is relatively safe when administrated orally in rats.In MCF 7 Cell line- LC50 Value NAGACHENDOORAM: 158.540698μg/mL (Calculated using ED50 PLUS V1.0 Software). In MDA MB 231 cell line-LC50 Value : 106.257379μg/mL (Calculated using ED50 PLUS V1.0 Software.in antioxidant activity of DPPH assay NAC shows 200 μg/ml shows that 43.35163 Percentage of inhibition. It has been showed that the NAAGA CHENDHOORAM is very effective in treating MULAI PUTRU (Breast Cancer) without causing any adverse effects. CONCLUSION: Management of cancer with a holistic approach, devoid of any side effects is now the major challenge to the medical system. This work highlights NAAGA CHENDHOORAM as novel anti cancer agent which provide a basis for the traditional use of it and proves that it could provide a cost effective and holistic remedy, without any side effects. From the literature review, physiochemical, pharmacological, microbiological, phytochemical, Instrumental analysis support the traditional use of NAAGA CHENDHOORAM in Siddha system of medicine for the treatment of breast cancer (mulai putru).
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | Reg.No.321912105 |
| Uncontrolled Keywords: | Scientific Validation, Siddha Herbomineral Formulation, Naaga Chendooram, Anti-Cancer Activity, Apoptosis inducing Potential, Anti Tumour, MCF-7, MDA-MB 231 Cell Lines, Antioxidant Activity, In-Vitro Methods. |
| Subjects: | AYUSH > Gunapadam |
| Depositing User: | Subramani R |
| Date Deposited: | 01 May 2023 13:00 |
| Last Modified: | 14 Feb 2024 14:54 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/21158 |
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