Bhoopathy, R (2021) Evaluation of Methanolic Extract of Sesbania Grandiflora Leaves for Anti-Migraine and Antioxidant Property in Nitroglycerin-Induced Migraine Rat Model. Masters thesis, C.L. Baid Metha College of Pharmacy, Chennai.
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Abstract
Effect of SGLE on Locomotor Behaviour: The group II animals showed a significant decrease in locomotor behaviour which involved walking and jumping when compared to Group I animals. Pre-treatment with SGLE [200 mg/kg (low dose) Group IV and 400 mg/kg Group V (High dose)] and standard treated with sumatriptan (Group III) significantly increased the locomotor activity on comparison with Group II. Effect of SGLE on Grooming Behaviour: The group II animals showed a significant increase in grooming behaviour which involved body grooming, scratching and licking of paws when compared to Group I animals. Pre-treatment with SGLE (Low 200 and High 400 mg/kg) (Group IV and Group V) and standard treated with sumatriptan (Group III) significantly decreased the grooming behaviour on comparison with Group II. Effect of SGLE on Rearing Behaviour: The group II animals showed a significant decrease in rearing behaviour which involved rearing and sniffing when compared to Group I animals. Pre-treatment with SGLE (Low 200 and High 400 mg/kg) (Group IV and Group V) and standard treated with sumatriptan (Group III) significantly increased the rearing behaviour on comparison with Group II. Effect of SGLE on Resting Behaviour: The group II animals showed a significant increase in resting behaviour which involved sleeping and hardly any movement when compared to Group I animals. Pre-treatment with SGLE (Low 200 and High 400 mg/kg) (Group IV and Group V) and standard treated with sumatriptan (Group III) significantly decreased the resting behaviour on comparison with Group II. Effect of SGLE on Analgesic Effect: The results of hot plate test significantly increased the pain threshold at 30, 60 and 90 minutes in high and low doses of SGLE and standard group when compared with the NTG treated group. The results of tail flick test significantly increased the tail flick latency in high and low doses of SGLE and standard group when compared with the NTG treated group. In Formalin test the paw licking frequency was significantly decreased in high and low doses of SGLE and standard group when compared with the NTG treated group. The Acetic acid induced writhing test shows less significance in the writhing number when compared with the standard group aspirin. In Light and Dark Box test time spent and the number of entries in light compartment in SGLE high and low dose group was significantly increased when compared with NTG treated group. Effect of SGLE on Serotonin (5-HT level): The brain level of 5-HT in groups of high and low dose of SGLE and standard was significantly increased when compared with the NTG treated group. Whereas NTG group showed significant decrease when compared with control group. Estimation of antioxidants enzymes: Effect of SGLE on Superoxide dismutase (SOD): SOD levels in brain of group II was significantly decreased when compared with group I (control) animals. Treatment group SGLE 200 and 400mg/kg showed a significant increase in SOD levels when compared with NTG group. Effect of SGLE on Glutathione Reductase: GSH levels in brain of group II was significantly decreased when compared with group I (control) animals. Treatment group SGLE 200 and 400mg/kg showed a significant increase in GSH levels when compared with NTG group. Effect of SGLE on catalase: Catalase levels in brain of group II was significantly decreased when compared with group I (control) animals. Treatment group SGLE 200 and 400mg/kg showed a significant increase in catalase levels when compared with NTG group. HISTOPATHOLOGY RESULTS: The drug treated animals and NTG treated animals brain was isolated and the cortex and hypothalamus was identified waxed and sliced in microtome, stained with eosin red. Control group: No pathological changes was noted in normal control. NTG Group: NTG induced group show pathological changes such as subpial congestion, perivascular lymphocytic infiltrate and mild gliosis. Standard: The congestion of lymphoid infiltrate after treatment with standard remained lesser when compared with the NTG group. Mild gliosis is reversed. Low dose SGLE: Low dose on comparison with NTG show marked reduction in pathological changes and mild congestion of lymphoid infiltrate is seen. High dose SGLE: High dose on comparison with NTG treated group shows a complete remission of pathological conditions noted in NTG group. CONCLUSION: For the above study it becomes evident that intraperitoneal injection of NTG produces migraine like pain response on rats. The SGLE pre-treated groups results suggest is beneficial in preventing migraine. The behavioural findings indicate SGLE is beneficial in controlling the psychological changes associated with migraine. The anti-migraine and anti-nociceptive effects of SGLE is confirmed from the neurotransmitter estimation of 5-HT and analgesic studies. The antioxidant property of SGLE also helps improve the antimigraine effect in which antioxidants act as neuroprotective. So further studies expected on isolated compounds of the extract in order to understand the exact mechanism and also receptor level studies are needed for investigation. Collectively we can say that herbal leaf extract of Sesbania Grandiflora demonstrated antioxidant, anti-migraine and improved behavioural changes in animal models of migraine which may deserve further evaluation through clinical studies.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 261925002 |
| Uncontrolled Keywords: | Methanolic Extract, Sesbania Grandiflora Leaves, Anti-Migraine, Antioxidant Property, Nitroglycerin-Induced Migraine Rat Model. |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Subramani R |
| Date Deposited: | 03 Dec 2022 08:42 |
| Last Modified: | 03 Dec 2022 08:42 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/21006 |
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