Srimyvizhiy, G M (2021) Development and Validation of UV-Multivariant Calibration Technique and HPTLC Method for the Estimation of Propafenone Hydrochloride in its Tablet Dosage Form. Masters thesis, Nandha College of Pharmacy, Erode.
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Abstract
The UV-Multivariant calibration techniques and sensitive HPTLC method have been developed for the determination of propafenone hydrochloride in its tablet dosage form. In UV-Multivariant calibration technique, ethanol was used as a solvent. The standard and sample solution were scanned at 247 nm and the concentration ranges are 4 – 20 µg/ml. The correlation coefficient values are R2 = 0.9998, LOD = 0.451, LOQ = 1.36.55 and the percentage purity of propafenone hydrochloride formulation were found to be 99.56 %. The developed UV method was validated as per the ICH guidelines. The observed values are within the acceptable limits. An optimized HPTLC method employing UV detection has also been developed and validated for the determination of propafenone in its tablet dosage form. In HPTLC – UV detection was observed at 251 nm. The pre-coated silica gel G60F254 used as a stationary phase and the mobile phase consisting Chloroform: Methanol: Acetic acid (8:1.5:0.5 v/v/v). The Rf value was calculated at 0.72. The linearity ranges are 200-1000 ng/spot for propafenone. The linearity data revealed that the developed method is linear. The correlation coefficient R2 was 0.9995. The precision and accuracy data lies within the reference range and the percentage label claim of propafenone hydrochloride was found to be 99.94 %. Both UV-multivariant calibration and HPTLC method can be used for routine analysis of propafenone hydrochloride in its tablet dosage form without any interference of excipients. The newly developed densitometric method having high efficiency and quantification with less concentration levels when compared to previous method.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 261930402 |
| Uncontrolled Keywords: | Development, Validation, UV-Multivariant Calibration Technique, HPTLC Method, Estimation, Propafenone Hydrochloride, Tablet Dosage Form. |
| Subjects: | PHARMACY > Pharmaceutical Analysis |
| Depositing User: | Subramani R |
| Date Deposited: | 03 Nov 2022 16:03 |
| Last Modified: | 03 Nov 2022 16:03 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/20951 |
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