Polyphenolics Screening in Mollungo Nudicaulis using UPLC-ESI-MS and Its Active Compound Kaempferol against SARs CoV2 Receptor

Kanmani, R (2021) Polyphenolics Screening in Mollungo Nudicaulis using UPLC-ESI-MS and Its Active Compound Kaempferol against SARs CoV2 Receptor. Masters thesis, J.K.K. Nattaraja College of Pharmacy, Kumarapalayam.


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Summarily, total phenolics and flavonoid content of MN 90% ethanolic extract was quantified through spectrophotometrically (50 ± 0.85 and 48.52 mg/kg), Higher flavonoid content plays major role on antioxidant activities, which were evaluated and compared with commercial antioxidant butylated hydroxytoluene (BHT) employing superoxide anion scavenging activity and total reducing power IC50 value results shows 152 and 135 μg/ml respectively. The extracted sample with known quantity (10μg/ml to 200μg/ml) used against E. coli, S. aureus and B. subtilis, MIC results shows 43.2 ± 3, 22 ± 2 and 21 ± 4 μg/ml respectively. The literature survey revealed that so far, no scientific studies carried out on phytochemical identification and confirmation using LC-MS analysis of Mollugo nudicaulis leaves. Hence, in the present study, we focused to evaluate the phytochemical content, docking and antimicrobial ability of Mollugo nudicaulis. In this study, aqueous and methanolic extracts of MN were taken for analysis, methanolic extract exhibited higher antioxidant activities in all the three antioxidant assays performed (ABTS, DPPH and reducing power). The antioxidant activities of methanolic extracts from the leaves and stems of were assessed in various herbal plants in an effort to compare and validate the medicinal potential by quantifying phenolic, flavanoid contents and by antioxidant assays such as DPPH, ABTS and ferric reducing power assays. Our results indicate that presence of significant quantity of total phenolis and flavonoids and antioxidant activity in MN leaves. This makes us to interpret that MN leaves possess promising health beneficial phytochemicals. It is widely accepted that phenolic and flavonoids compounds may significantly contribute to overall antioxidant activities and also to antimicrobial activity. Ok-Hwan Lee and Boo-Yong Lee have purified the phenolic compounds from olive leaves and tested them for antioxidant and antimicrobial activities. Methanolic extract of MN leaves showed antibacterial activity against all the four bacterial species tested such as Pseudomonas aeruginosa, Proteus sp, Streptococcus sp and Entrobacter sp. Similar studies were carried out in Merremia emarginata leaves extracts against S. aureus, Staphylococcus epidermidis, E. coli, and P. aeruginosa to test the antibacterial activity. The abovesaid biological properties were significantly attributed to the presence of bioactive compounds of the M. nudicaulis which have been revelaed through LC-MS/MS analysis. Moreover, their activities have been further proved through in-silico molecular docking studies in which major bioactive compounds such as Rutin, Physcion 8-O-glucoside, and Kaempferol 3-O-glucuronide showed effective inhibitory interaction with SARs CoV2 receptor. This altogether suggests that M. nudicaulis can be a potential source of antioxidants and antimicrobial compounds to combat pandemic viruses. CONCLUSION: The maximum yield of phenolics and flavonoids content shows method suitability of flavonoids extraction and quantification. Further, plant extract versus anti-oxidant and its role against SARs Cov2 receptor. Further, the receptor binding possibilities was confirmed using molecular docking study against Kaempferol. results conclude plant extract were act as good anti-oxidant and anti-microbial agent. Based on our findings we conclude that Mollugo nudicaulis have significant amount of total phenolics and flavonoids. Methanolic extract of Mollugo nudicaulis has shown higher in invitro antioxidant and antimicrobial activity. Further work will be carried out to find biologically active compounds purification and structural elucidation through NMR studies.

Item Type: Thesis (Masters)
Additional Information: 261915205
Uncontrolled Keywords: Polyphenolics Screening, Mollungo Nudicaulis, UPLC-ESI-MS, Active Compound, Kaempferol, SARs CoV2 Receptor.
Subjects: PHARMACY > Pharmaceutical Chemistry
Depositing User: Subramani R
Date Deposited: 02 Nov 2022 17:35
Last Modified: 02 Nov 2022 18:13
URI: http://repository-tnmgrmu.ac.in/id/eprint/20921

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