Punith Kumar, C R (2021) Anti-Tumor Potential of Seed Kernels of caesalpinia bonducella extracts and Isolated Phytoconstituents: In Vitro and In Silico Analysis. Masters thesis, C. L. Baid Metha College of Pharmacy, Chennai.
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Abstract
Although there are several allopathic drugs available, many people still rely on nature that has blessed the human species with abundant herbs. The occurrence of cervical cancer is still increasing worldwide. In India, cervical cancer contributes to approximately 6–29% of all cancers in women. It is estimated that cervical cancer will occur in approximately 1 in 53 Indian women during their lifetime. Apart from HPV Vaccine (human papillomavirus vaccine) the standard drugs used for treatment are cisplatin, carboplatin, etc. Although with the advent of new synthetic drugs, the side effects (tolerable or severe) on chronic use have led us to look for effective drugs with less or no side effects. Therefore, there is an attempt to evaluate the traditional claims of Caesalpinia bonducella seed kernels for the treatment of cervical cancer. The review on literature showed other works have been done on this plant but still activity against cervical cancer has not been reported so far. The project entitled Anti-tumor potential of seed kernels of Caesalpinia bonducella(Fabaceae)extracts and isolated phytoconstituents: in vitro and in silico analysis has been achieved with the following results. Macroscopical studies states the characteristic features of Caesalpinia bonducella seeds such as globular shape, characteristic odour, green in colour and other morphological characters has been well studied and established. Microscopic investigations are the diagnostic features and its powder analysis helps to distinguish this particular plant (leaves) in whole form and in crude powder form. Microscopical analysis in Caesalpinia bonducella showed the presence of schlerenchymatous fibres, Starch grains, Parenchyma cells, Oil Globules, Stone cells and calcium oxalate crystals. The above findings help to bring the identity determined the leaves by both morphology and anatomy. Various physicochemical constants were determined for the seeds of Caesalpinia bonducella such as Ash values, Extractive values and loss on drying. This helps in confirming the identity and purity of this plant and to detect adulterants and its nature. The phytochemical studies on the seeds of Caesalpinia bonducella were carried out to bring its importance as a valuable medicinal plant. The total methanolic extract was carried out using Hot continuous extraction apparatus. The percentage yield of methanolic extract was 8.3% w/w. The extract was subjected to preliminary phytochemical screening which revealed the presence of Flavonoids, Phenols, Saponins, Terpenoids, Alkaloids, Cardiac Glycosides and Tannins. Quantification of phytoconstituents was done for flavonoids and phenolic compounds by UV spectroscopy method. The obtained extract was enriched in flavonoid content by removing all other phytochemicals present in it by partition chromatography. Phytochemical screening of the enriched extract revealed the presence of Flavonoids, Phenols and Cardiac glycosides. Also, the methanolic extract was subjected to column chromatography which resulted in the isolation of compounds Quercetin and Kaempferol which was confirmed in the later stage by FT-IR and Mass spectroscopy. Thin Layer Chromatography was performed for the enriched extract and both the isolated compounds. In vitro pharmacological studies, MTT assay on HeLa cells against cervical cancer cell lines were performed for the enriched extract to find out the anti-cancer activity. Also it has been tested for Anti-bacterial and Anti-fungal activity. In silico docking analysis were performed on CDK 6 enzyme (1XO2) by the isolated constituents of Caesalpinia bonducella to evaluate the anti-cancer activity using molecular docking analysis. The Insilico studies revealed that both the compounds, Quercetin and Kaempferol, possessed significant binding energy and inhibition constant values. Hence from the studies it can be concluded that the seed kernels of Caesalpinia bonducella possess significant activity against cervical cancer. This investigation has a potential to be developed further into a natural Anti-cancer drug. Further detailed studies on more flavonoids and especially Homo isoflavonoids (HIFs) which is a part not much explored by many has to be studied.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 261915009 |
| Uncontrolled Keywords: | Anti-Tumor Potential, Seed Kernels, caesalpinia bonducella extracts, Isolated Phytoconstituents, In Vitro and In Silico Analysis. |
| Subjects: | PHARMACY > Pharmaceutical Chemistry |
| Depositing User: | Subramani R |
| Date Deposited: | 02 Nov 2022 17:09 |
| Last Modified: | 04 Nov 2022 11:16 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/20910 |
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