Sruthi, R (2021) Enhancement of Wound Healing activity of Papain Urea Film Forming Hydrogel using Chitosan. Masters thesis, Periyar College of Pharmaceutical Sciences, Tiruchirappalli.
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Abstract
The film-forming hydrogel (FFH) is a hydrogel dosage form which transform from the hydrogel to film-type by solvent evaporation after application to the wound site. Over time, the film erodes and is spontaneously removed. Such a film-forming gel shares many protective advantages with the skin barrier itself (e.g., confers protection to the treatment site from environmental threats and is resistant to washout), but also possesses distinct advantages of a gel (e.g., easy application and good adhesiveness to treatment sites). Compared with wound dressing forms, it offers easier use and application, and simpler manufacture. The FFH system can be freely applied to any wound site, even though the wound is curved and shaped. Furthermore, the FFH system was hardly attempted to be developed as a pharmaceutical product for wound healing. The λ max of Papain was found to be 277.4nm in phosphate buffer pH 7.4. Papain obeys Beer’s law within concentration 10- 60μg/ml FTIR studies showed that there was no interaction between drugs and excipients The film forming hydrogel was prepared by simple agitation method using different concentration of polymer. The Pre formulation parameters were within the required limit, indicating suitable to formulate film forming hydrogel The Post formulation parameters such pH, viscosity, spreadability, homogeneity, extrudability, drug content, drying time of all the formulation was within the acceptable limits. F6 formulation was selected as the best formulation based on in vitro release studies. The extent of drug release was found to be 98.19% in 12 hours. The drug release follows zero order, non fickian diffusion mechanism. Cell cytotoxicity study was done in optimized formulation. This assay was helped in dose fixation for in vitro wound healing activity. It shows cell viability >50% in concentration 10- 500μg/ml, indicating presence of non skin irritants. In vitro wound healing activity was evaluated in Vero cell lines and the formulation enhanced the wound closure in Vero cells. Optimized formulation showed controlled release profile than the marketed conventional formulation. There was no significant change before and after stability study. CONCLUSION: For controlled drug delivery, a topical route is the most popular and successful route. The goal of this work is to develop Papain urea containing film-forming gel for prolonged release based on topical delivery and to treat wound healing in a short duration of time. Film forming gels also proves to be an efficient dosage method for the topical delivery of wound healing. It also stays adhered to the affected portion for a longer time without being rubbed off. It offers prolonged effect than conventional dosage forms, and also there is no need for regular re- application. Since the formulation is prepared with a drug and also the polymer having wound healing effects, the wound healing efficiency will be in higher rate. The prepared topical drug delivery system of Papain urea using chitosan as a polymer was a gel before administration, and forms film after application. The idea will change the therapy of different diseases and provide young researchers and scientists with a broad platform in the field of film forming gel.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 261910205 |
| Uncontrolled Keywords: | Enhancement, Wound Healing activity, Papain Urea Film, Forming, Hydrogel, Chitosan. |
| Subjects: | PHARMACY > Pharmaceutics |
| Depositing User: | Subramani R |
| Date Deposited: | 01 Nov 2022 16:21 |
| Last Modified: | 01 Nov 2022 16:21 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/20874 |
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