Characterization, Docking Studies and Anticancer Potential of Silver Nanoparticals in Ethanolic Extract of Sargasssum Polycystum C.Agardh

Mari Ganesh, M (2021) Characterization, Docking Studies and Anticancer Potential of Silver Nanoparticals in Ethanolic Extract of Sargasssum Polycystum C.Agardh. Masters thesis, Arulmigu Kalasalingam College of Pharmacy, Krishnankoil.


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Abstract: The brown algae of Sargassum Polycystum C.Agradh. were collected from mandapam camp (18 km from the Indian coast), Ramanathapuram Dist, Tamilnadu, India. The dried samples were authedicated by Principal Scientist, Central Marine Fisheries Research Institute, Mandapam Regional Centre of CMFRI, Mandapam Camp-623 520. The specimen voucher was deposited in Research Lab, Arulmigu Kalasalinagm College of Pharmacy, Srivilliputtur. The dried samples (Sargassum Polycystum C.Agradh) was carried out to prepared ethanolic extract and the extact were subjected to preliminary phytochemical screening including solubility test, ash test. Followed the crude extract was characterized by TLC and it confirmed by Uv-Visble spectral analysis. The crude extract is characterized qualitatively by using hyphenated technique GC-MS. The report revealed it contains 5 different compounds such as 1. phenol2,4-bis-(1,1 dimethyl),2. Methyltris (trimethylsilooxy)silane, 3. 1, 2 bis(trimethylsilyl)benzene, 4. Trimethyl(4-tert-butylphenoxy)silane, 5. Phenol,2,5-bis(1,1-dimethylethyl) and that will carried out hplc analysis in order to find out the possible phyto constituents we understand that it contains phenolics such as gallic acid, p-hydroxybenzoic acid, vanillic acid, pcoumaric acid and ferulic acid with a distinct peak. FTIR results revealed that one strong adsoption showed at 3307cm-1 assigned for OH stretching. One strong adsoption showed at 2943cm-1 assinged for CH Stretching (Aromatic) One strong adsoption showed at 2831cm-1 assinged for CH Stretching (Aliphatic) One strong adsoption showed at 1111cm-1 assinged for CH bending. 1H NMR (400 MHZ, DMSO, PPM): One signal showed at 2.3 d assigned for methyl proton. One signal showed at 3.6 t assigned for CH2 Proton. signal showed at 6.5 to 8.2 m assigned for aromatic proton. One signal showed at 10.1 assinged for aliphatic aldehyde proton. The obtained crude extract was carried out in to silver nano particles. The prepared silver nano aprticles were analysed by scanning electron microscope (SEM). The crude extract as well as silver nano particles were subjected to anti-cancer activity against HT29 (Human colorectal adenocarcinoma) cell line by MTT assay method, 5- fluorouracil (5-FU) using as standard. It was found to be IC50 value 199.67 μg (crude extract) and 144.30 μg (silver nano particles) against standard 2.71 μg (5FU). Further the active compound was subjected to In vivo chorioallantoic membrane (CAM) assay for the understanding of anti-antiangiogenisis activity with 5 days embryo eggs. From the study it found to the compound inhibit the formation of blood-vessels, Imaging of the vascularized eggs was performed using a digital camera with 3x magnification Objective. They above results show ethanolic extract of Sargassum Polycystum C.Agradh have moderate anti cancer potential, this was determined may due to the presence fucoidan as a one of the important component of this extract . In order to proven this, the obtained extract was subject to docking studies by Molegro Virtual Docker Evaluation Version (MVD 2013.6.0) docking method against EGFR (Epidermal growth factor receptor)PDB code 1m17 and ligand (fucoidan) From the docking result reveals two amino acids(thr 766, lys 721) are involve in hydrogen bonding interaction and three amino acids( lys 721, thr 766, Asp 831) are involved Electrostatic interaction in ligand (fucoidan)-protien (1m17) complex moreover docking studies reveals amino such as eight amino acids (Asp 831, Lys 721, Ala 719, Thr 766, leu 764, Met 742, Thr 830,Glu 738) involved in this steric interaction of ligand protein complex. From the study, finally it concludes marine seaweed (brown algae) contains rich amount of minerals, phenolic compounds, moreover it have potent active against anticancer. Further research will plan to execute for in order getting future fruit full scope in the field marine.

Item Type: Thesis (Masters)
Additional Information: 261915351
Uncontrolled Keywords: Characterization, Docking Studies, Anticancer Potential, Silver Nanoparticals, Ethanolic Extract, Sargasssum Polycystum C. Agardh.
Subjects: PHARMACY > Pharmaceutical Chemistry
Depositing User: Thavamani K
Date Deposited: 12 Aug 2022 02:04
Last Modified: 16 Aug 2022 05:14

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