Kalaivani, V (2021) Study of Phytochemical Analysis and Invitro Anti-Diabetic, Anti-Inflammatory Activitys of Cyperus Rotundus. Masters thesis, Annai J.K.K. Sampoorani Ammal College of Pharmacy, Komarapalayam.
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Abstract
Our study clearly shows that Cyperus rotundus caused marked hypoglycemic activity in alloxan induced diabetic rat model which indicates anti-diabetic potentials of the methanol extract and test isolated compounds. The compound Quercetin-3-O-rutinoside-7-O-rhamnoside isolated from Cyperus rotundus tubers tested for anti-diabetic activity as it significantly lowered the serum glucose levels and increased the body weight of diabetic rats. In this experiment, the dose of 200 mg / kg produced the highest anti-diabetic effect and this may suggest that this dose may be the effective anti-diabetic drug even though there might have some limitation due to the crude nature of the extract. The results here demonstrate for the first time the topical anti-inflammatory efficacy of cyperus rotundus extract suggesting that the extract could be a potential new therapeutic tool for the treatment of inflammatory skin disorders. Summary: The present work is to isolate a flavonoid glycoside from tubers of Cyperus rotundus using chromatography separation techniques, spectral characterization and its anti-diabetic activity. The structure of the isolated compound was purified, analyzed and characterized by chemical tests, HPLC and spectroscopic methods such as UV, IR, 1H-NMR and 13C-NMR. The blood sugar level was measured by digital display glucometer. The HPLC analysis showed the presence of a flavonol glycoside and it’s identified peak with the retention time of 24.8 min and exhibited two major peaks at 360 nm (band-I) and 261 nm (band-II). The tubers of Cyperus rotundus have been found to contain Quercetin-3-O-rutinoside-7- O-rhamnoside. The highest activity of Cyperus rotundus tubers extract in this experiment was observed at the dose of 200 mg / kg of isolated compound while the reference drug glibenclamide (10 mg/kg) had a superior activity when compared with Cyperus rotundus tubers extract. The topical anti-inflammatory efficacy of cyperus rotundus extract suggesting that the extract could be a potential new therapeutic tool for the treatment of inflammatory skin disorders.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 261915602 |
| Uncontrolled Keywords: | Phytochemical Analysis, Invitro Anti-Diabetic, Anti-Inflammatory Activitys, Cyperus Rotundus. |
| Subjects: | PHARMACY > Pharmaceutical Chemistry |
| Depositing User: | Thavamani K |
| Date Deposited: | 08 Aug 2022 12:13 |
| Last Modified: | 18 Aug 2022 10:54 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/20698 |
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