Isolation, Characterization and Evaluation of Antidiabetic Activity on Leaves of Merremia Hederacea (Burm. F.) Hallier F.

Yuvaraj, B (2019) Isolation, Characterization and Evaluation of Antidiabetic Activity on Leaves of Merremia Hederacea (Burm. F.) Hallier F. Masters thesis, College of Pharmacy, Madras Medical College, Chennai.

[img]
Preview
Text
261218419yuvaraj.pdf

Download (7MB) | Preview

Abstract

The global human population emerges to be in the midst of an epidemic of diabetes. In spite of the enormous steps that have been made in the perceptive and executive of diabetes, the disease and disease related problems are increasing spontaneously. Equivalent to this, current investigations and studies in understanding the pathophysiology of the disease process have opened up numerous novel avenues to recognize and expand new therapies to fight the diabetic condition. Simultaneously, phytochemicals bioactive compounds identified from traditional medicinal plants are presenting an exhilarating prospect for the improvement of novel therapeutics. This has step up the world wide effort to exploit and collect those medicinal plants that bear considerable amount of potential phytochemicals presenting multiple beneficial effects in combating with diabetes and diabetes-related difficulties. Therefore, as the disease is progressing alarming rates, there is an urgent need of identifying indigenous natural resources in order to procure them, and study in detail, their potential on different newly identified targets in order to develop them as new therapeutics. In these regard we have made an attempt to investigate the in vitro and in vivo anti diabetic activity of Merremia hederacea. The isolated pure compound has been attempted for molecular docking studies. From all these observations it is concluded that ethanol extract of Merremia hederacea possess significant antidiabetic activity. The concentration of blood glucose was significantly increased in STZ induced diabetic as compared to normal control. Administration of ETMH (200 mg/kg and 400 mg/kg) significantly reduced the raised blood glucose level in STZ induced diabetic rats and the lowering was almost comparable to glibenclamide 5mg/kg.). The blood glucose lowering effect of the ETMH could be due to the presence of novel chalcone (2',4,4'-trihydroxy-3'–(methyl-2-butenyl) chalcone) which is isolated from the leaf of this plant by column chromatography in this study. The isolated compound is characterized by HPLC/PDA/MS, 1HNMR & IR. From the observations of molecular docking studies and in vitro anti diabetic and anti cancer activity of the isolated compound (2',4,4'-trihydroxy-3'–(methyl-2-butenyl) chalcone) it has been concluded that chalcones are, undoubtedly, promising anti-diabetic agents, and some crucial structural features have already been CONCLUSION Department Of Pharmaceutical Chemistry, MMC Page 82 established. From the structure-activity relationships analysis, it can generally be stated that the presence of hydroxyl, prenyl and geranyl groups in their skeleton improves their activity for the evaluated antidiabetic targets. The compound shows greater percentage inhibition for alpha glucosidase at 320μg compared to standard and has a good docking score of -11.28 Kcal-1 against diabetic target protein, alpha glucosidase. The 2′, 4, 4′ -Trihydroxy-3′-(3-methyl-2- butenyl) chalcone shows good anticancer activity against liver cancer cell line (HepG2) compared to the standard 5 fluro Uracil. The compound has a docking score of – 9.56 kcal/mol against cancer target protein. FUTURE PROSPECTS  The isolated 2, 4, 4′ -Trihydroxy-3′-(3-methyl-2-butenyl) chalcone will be focused for lipid encapsulation nanotechnology and will be investigated in future for effective drug delivery system.  The isolated 2, 4, 4′ -Trihydroxy-3′-(3-methyl-2-butenyl)chalcone will be evaluated in future for enzyme inhibition assay of Protein tyrosine Phosphatase 1b ( the diabetic target protein).  The isolated 2, 4, 4′ -Trihydroxy-3′-(3-methyl-2-butenyl)chalcone will be evaluated in future for in vitro anti tubercular activity.

Item Type: Thesis (Masters)
Additional Information: 261715709
Uncontrolled Keywords: Isolation, Characterization, Antidiabetic Activity, Leaves, Merremia Hederacea (Burm. F.) Hallier F.
Subjects: PHARMACY > Pharmaceutical Chemistry
Depositing User: Ramakrishnan J
Date Deposited: 10 May 2022 09:38
Last Modified: 10 May 2022 09:38
URI: http://repository-tnmgrmu.ac.in/id/eprint/20012

Actions (login required)

View Item View Item