Megala, S (2019) Pharmacognostical, Phytochemical Studies and Evaluation of Anti-Arthritic Activity of Leaves of Momordica Charantia Linn.,. Masters thesis, College of Pharmacy, Madras Medical College, Chennai.
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Abstract
The pharmacognostical studies on the leaves of Momordica charantia Linn., was carried out, which showed the unique features of the leaves which is used to differentiate it from other species. Macroscopical studies states the characteristic features of the leaves like green colour, intensely bitter in taste and had characteristic odour. The leaves are simple and alternate, smooth and thick. Anatomical studies of leaves showed the presence of epidermis, palisade parenchyma, spongy parenchyma and vascular bundles. Powder analysis of the leaves showed the presence of non glandular trichomes, anomocytic stomata, idioblasts, fibres and palisade cells. Linear measurement for trichomes and leaf constant of stomatal number, stomatal index, vein islet number and vein termination number were evaluated for the leaves of Momordica charantia Linn., Physiochemical studies: Various physiochemical constants were evaluated such as ash values, extractive values, loss on drying, foaming index and swelling index. These pharmacognostical parameters evaluated are useful for the establishment of standards of leaves which essential for its identity and purity. PHYTOCHEMICAL STUDIES In phytochemical study, the powdered leaves is successively extracted with nhexane, chloroform, ethyl acetate, ethanol and aqueous by using soxhlet apparatus. Preliminary phytochemical studies was done for the powdered leaves and all the extracts. It was found to contain flavonoids, saponins, alkaloids, triterpenoids, phenolic compounds, steroids, etc., Quantitative estimation of the phytoconstituents was carried out for phenolic compounds, flavonoids and saponins. Fluorescence analysis was done to find out characteristic fluorescent substance present in the powdered leaves and all the extracts, and no fluorescent substance was found. IR spectrum analysis was carried out for identify the functional groups present in ethanolic extract of leaves of Momordica charantia Linn., TLC analysis of leaves extract and HPTLC analysis of ethanolic extracts were carried out to identify phytoconstituents present. PHARMACOLOGICAL STUDIES Invitro studies All the extracts were subjected to invitro anti arthritic activity to select the most bioactive extract. Based on this ethanolic extract showed maximum inhibition of protein denaturation and membrane stabilization activity. Hence the ethanolic extract was selected for the invivo studies. Invivo studies Arthritic activity was assessed by method of Complete Freund’s adjuvant induced arthritis. The ethanolic extract of dose 200 and 400mg/kg were given orally to the rats, it is compared with standard Diclofenac sodium. The parameters studied were rat paw swelling, body weight changes, hematological parameters and histopathological studies. The ethanolic extract at the dose 400mg/kg showed significant anti-arthritic effect on the arthritis induced rats which was comparable with the standard.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 261720652 |
| Uncontrolled Keywords: | Pharmacognostical, Phytochemical Studies, Anti-Arthritic Activity, Leaves, Momordica Charantia Linn., |
| Subjects: | PHARMACY > Pharmacognosy |
| Depositing User: | Ramakrishnan J |
| Date Deposited: | 10 May 2022 07:32 |
| Last Modified: | 10 May 2022 07:32 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/20008 |
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