Srividhya, S (2022) Comparative Evaluation of Viability of Human Periodontal Ligament Fibroblasts (PDLF) in Six Different Storage Media at Four Different Time Intervals: An In-Vitro study. Masters thesis, Chettinad Dental College and Research Institute, Kanchipuram.
Full text not available from this repository.Abstract
AIM OF THE STUDY: To compare and evaluate the viability of Human Periodontal ligament fibroblasts (PDLF), in six varied storage media solutions at four different time intervals. MATERIALS AND METHODS: To evaluate the viability, human periodontal ligament fibroblast cell line (L929) was procured and the cells were cultured in the 96-well culture plates. The six various storage media testing solutions which include Hank’s balanced salt solution (HBSS), milk, tender coconut, oral rehydration solution (ORS), egg white, and tap water were deposited into the culture plates consisting of the fibroblasts. The above-mentioned setup is incubated at 37 degrees C in 5% CO2 and then the cell viability analysis was done with the help of 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The viability of the cells (in percentage) was estimated at the end of four varied time intervals- ½ hour, 1 hour, 3 hours, and 6 hours. The mean and standard deviation of the viable cells were evaluated statistically using one-way ANOVA and significance was established. RESULTS: The viability of human PDL cells at ½ hour and 6-hour time intervals were 98.3% and 26.4% for group I (Tender coconut) and 91.8% and 8.8% for group II (Milk) respectively. The viability of human PDL cells for group V(HBSS) was 92.6% and 18.4% at ½ hour and 6-hour time periods respectively. 86.1% and 7.2%, 96.7% and 12.8% were the percentage viability shown by the group-IV and VI i.e., oral rehydration solution (ORS) and egg white at ½ and 6-hour time intervals respectively. The tap water (group III) showed the least % viability of 83.7% and 7.2% at both time intervals. One-way ANOVA reveals a statistical significance (0.00) at 4 different time intervals within the groups. CONCLUSION: Tender coconut showed the highest % viability of cells not only at ½ hour time interval but also at the end of the 6th hour. The performance of egg white though is better at ½ hour interval performs lesser at 6th-hour interval in comparison to the HBSS. However, the efficiency of tender coconut in proving its ability to maintain cell viability throughout the varied time intervals is considerable.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 241917752 |
| Uncontrolled Keywords: | Cell viability, MTT assay, Periodontal ligament fibroblasts, Storage media, Tooth avulsion, Tender coconut. |
| Subjects: | DENTAL > Conservative Dentistry and Endodontics |
| Depositing User: | Subramani R |
| Date Deposited: | 08 May 2022 12:59 |
| Last Modified: | 13 May 2022 15:28 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19852 |
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