Sulmuna, R (2022) Immunohistochemical Evaluation of Cancer Stem Cell SOX2 and Tumor Suppressor Protein p53 in Oral Squamous Cell Carcinoma: A Case Control study. Masters thesis, Rajas Dental College and Hospital, Tirunelveli.
Full text not available from this repository.Abstract
BACKGROUND: Oral squamous cell carcinoma is the sixth most prevalent malignancy reported worldwide. It accounts for 45% of all cancers reported in India. One of the main characteristic clinical features of oral squamous cell carcinoma is invasion and metastasis to the adjacent tissues. It is a state of tumor heterogenicity where there is presence of cancer stem cells & mutated tumor suppressor genes. Biological markers are important to identify the progression of the tumor and management. SOX2 protein is a high mobility SRY (sex determining region Y) related HMG(homologues) box transcription factor, which regulates stem cells. SOX2 – essential for maintaining the self- renewal or pluripotency of undifferentiated embryonic stem cells. p53 is a tumor suppressor gene that plays an important role in cell cycle control & apoptotic response to effects of cell damage. p53 has a regulatory effect on SOX2 AIM OF THE STUDY: The aim of this study is to assess the expression cancer stem cell SOX2 and tumor suppressor protein p53 in oral squamous cell carcinoma cases compared to controls. OBJECTIVES: To evaluate the expression, of SOX2 and p53 in cases and controls. To compare the expression of SOX2 and p53 between cases and controls. To correlate the expression of SOX 2 and p53 with histopathological grades of OSCC. HYPOTHESIS: There is an alteration in the expression of SOX2 and p53 in subjects with oral squamous cell carcinoma cases compared to controls. MATERIALS & METHODS: Paraffin embedded tissue sections of OSCC(n=30), which include 10 well differentiated squamous cell carcinoma, 10 moderately differentiated squamous cell carcinoma and 10 poorly differentiated squamous cell carcinoma and normal mucosa (n=10) were confirmed histopathologically. The sections were stained with antibodies against SOX2 and p53. RESULTS: There was a significant increase in immunohistochemical expression of both SOX2 & p53 in oral squamous cell carcinoma among cases compared to controls. There is no association between histopathological grades of oral squamous cell carcinoma with thee expression of SOX2 & p53. There was no significant expression of SOX2 and p53 at the periphery of tumor islands. CONCLUSION: Increased expression of SOX2 in squamous cell carcinoma suggest a possible role in progression of the neoplastic cells. Increased expression of p53 in oral squamous cell carcinoma probably denoting mutant p53 expression and an increased malignant transformation potential. There is a significant correlation between the expression of p53 & SOX2. Therefore, SOX2 & p53 can probably be used as a prognostic marker for targeted therapy.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 241921552 |
| Uncontrolled Keywords: | Cancer stem cell, SOX2, Tumor suppressor protein p53, oral squamous cell carcinoma, Immunohistochemistry. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 08 May 2022 09:09 |
| Last Modified: | 14 May 2022 07:28 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19787 |
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