Comparative Evaluation of Twist and P53 in Oral Squamous Cell Carcinoma: An Immunohistochemical study

Aarthi Pandian, (2022) Comparative Evaluation of Twist and P53 in Oral Squamous Cell Carcinoma: An Immunohistochemical study. Masters thesis, Sree Mookambika Institute of Dental Sciences, Kulasekharam.

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BACKGROUND: Oral squamous cell carcinoma (OSCC) is a fatal and deforming disease of increasing incidences. The epithelial-mesenchymal transition (EMT) is a critical cellular program that enables polarized epithelial cells to change into mesenchymal phenotype with greater cellular mobility. The transcription factor TWIST plays a key role in the progression of the primary tumor to the stage of metastasis through the process of epithelial-mesenchymal transition. AIMS AND OBJECTIVES: The current study assessed and compared the expression of TWIST and p53 in oral squamous cell carcinoma cases with normal oral mucosa and investigate the possible correlation of their expression in different histopathological grades of oral squamous cell carcinoma. MATERIAL AND METHODS: A total of forty–five samples consisting of thirty paraffin-embedded tissue samples of oral squamous cell carcinoma cases and fifteen samples of normal oral mucosa was included in the study. Two sections each of the samples was taken on positively charged slides and were immunostained with TWIST and p53 respectively. RESULTS: The mean immunoexpression of TWIST and p53 between the oral squamous cell carcinoma and normal mucosa showed a significant variation with the highest values in squamous cell carcinoma cases. There was no statistical significance observed between different grades of oral squamous cell carcinoma but the mean value was increased in poorly differentiated squamous cell carcinoma respectively. CONCLUSION: A significant increase in TWIST and P53 in oral squamous cell carcinoma suggests their role in the malignant transformation of the oral epithelium. Therefore, TWIST and p53 might be an important target for therapeutic approaches in cancer patients, which require further investigations by long-term follow-up analysis.

Item Type: Thesis (Masters)
Additional Information: 241921301
Uncontrolled Keywords: Immunohistochemistry, Oral cancer, Oral squamous cell carcinoma, P53, TWIST.
Subjects: DENTAL > Oral Pathology and Microbiology
Depositing User: Subramani R
Date Deposited: 08 May 2022 08:55
Last Modified: 14 May 2022 06:59

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