Cibishalini, T (2022) Cytomorphometric and Cytogenetic Analysis of Exfoliated Buccal Mucosal Cells among Type 2 Diabetes Mellitus Patients with Tobacco Chewing and Arecanut Habits. Masters thesis, Vivekananda Dental College For Women, Tiruchengode.
Full text not available from this repository.Abstract
INTRODUCTION AND AIM OF THE STUDY: Diabetes mellitus is the group of metabolic disease characterized by hyperglycemia and resulting from the combination of resistance to insulin action, inadequate insulin secretion and excessive or inappropriate glucagon secretion. Tobacco in any form and arecanut chewing are considered to be the major extrinsic etiological factor for premalignant and potentially malignant disorders. The habit of tobacco and arecanut chewing has been reported to be associated with cancers of the mouth, pharyngeal cavity and upper part of the digestive tract. This study analyses the cytomorphometric and cytogenetic changes between Type 2 diabetes mellitus (T2DM) with tobacco and arecanut chewing habits using exfoliative cytology. METHODOLOGY: The study consists of three groups. Group 1 - 60 T2DM individuals with tobacco chewing habit, Group II - 60 T2DM individuals with arecanut chewing habit and Group III - 60 60 T2DM individuals without any habits. Cytological smears were prepared, staining done with RAPID PAP and analysis on Cytomorphometric [nuclear diameter (ND), cellular diameter (CD), and nucleus to cell ratio (NCR)] and cytogenetic [frequency of micronuclei (MN)] alteration were studied. Comparisons between Group I vs Group II, Group I vs Group III and Group II vs Group III were made. The interpretation was statistically evaluated using SPSS version 21 (IBM CORP, Chicago, IL, USA). The level of significance (p<0.05) was employed in all statistical comparisons. Quantitative data were recorded as mean ± standard deviation. Data were subjected to ANOVA TEST and TUKEY’S POST HOC MULTIPLE COMPARISON TEST. RESULTS: Group I showed a mean ND of 5.40± 1.35, Group II showed mean ND OF 4.39±0.96, and Group III showed mean ND of 3.86±0.50. The ND was increased in the study groups, (Group I and Group II) in comparison with control group (Group III). Group I showed a mean CD of 26.30 ± 3.41, Group II showed a mean CD of 32.06±3.85, Group III showed a mean CD of 34.34±2.84. The CD was decreased in the study groups, (Group I and Group II) in comparison with control group (Group III). The NCR showed significant difference between the study groups, (Group I and Group II). Group I showed a mean frequency of MN 1.04±0.12, Group II showed a mean frequency of MN 0.95±0.12, and Group III showed a mean of 0.82±0.20. Among the study groups, Group I show increased frequency of MN in comparison with that Group II. CONCLUSIONS: Significant increase in cytomorphometric and cytogenetic changes were observed in Type 2 DM tobacco chewers when compared with Type 2 DM arecanut chewers. Awareness should be created among Type 2 DM individuals, as they possess high risk of genetic damage and malignant transformation when they are exposed to tobacco chewers and arecanut habits comparing to non-diabetic individuals.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 241921451 |
| Uncontrolled Keywords: | Genetic damage, arecanut, tobacco, betel quid, oral cancer, micronuclei. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 08 May 2022 08:51 |
| Last Modified: | 14 May 2022 06:52 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19779 |
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