Evaluation of BCL2 and GLUT1 Expression in Oral Potential Malignant Disorder and Oral Squamous Cell Carcinoma

Prisilla, M (2022) Evaluation of BCL2 and GLUT1 Expression in Oral Potential Malignant Disorder and Oral Squamous Cell Carcinoma. Masters thesis, Chettinad Dental College and Research Institute, Kanchipuram.

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BACKGROUND: Oral cancer is the sixth most common cancer worldwide, 90% of which constitutes oral squamous cell carcinoma. Although it may arise denovo, 3 - 50 % of the histologically proven severe epithelial dysplasia amongst the potentially malignant disorders transform to malignancies. Immunohistochemical studies have been extensively done to study the various molecular aspects of disease process that aid in diagnosis and prognosis of tumourogenic behavior & survival rate. Glut1 is a glucose transporter protein and its expression is upregulated in malignant cells indicating increased glucose uptake. Glut 1 positivity help in proliferation by promoting hypoxic cells to undergo anaerobic glycolysis which in turn produce more glucose that ultimately influence the tumour cell survival rate and aggressive behavior of the tumour. Studies consider Glut 1 to be a biomarker in patients with head and neck squamous cell carcinoma. Bcl 2 is a protooncogene which directly or indirectly inhibit the release of cytochrome C thus preventing the activation of caspases and hence inhibiting apoptosis. This lead to cellular immortalization contributing to the formation of the tumour. Thus this study aim to evaluating the coexpression of Glut 1 and Bcl2 in oral squamous cell carcinoma immunohistochemically and also to assess whether they could serve as a biomarker in Head and Neck Squamous cell carcinoma. AIM: To evaluate the expression of Bcl2 and Glut1 in Oral squamous cell carcinoma using immunohistochemistry. MATERIALS AND METHOD: A total of 92 subjects were examined for analyzing the immunohistochemical expression of Bcl2 and Glut 1. The control group include 5 blocks of paraffin embedded tissue samples of normal buccal mucosa and the study group include 18 Oral potential malignant disorder subjects, 23 Oral squamous cell carcinoma subjects. Microscopic sections of 4 -5micron thickness were made from each samples and these samples were stained immunohistochemically using Bcl 2 and Glut 1to investigate its expression and staining intensity based on which the immunoreactive score( IRS) were calculated. The IRS were correlated with various clinical parameters to understand its significance. STATISTICAL ANALYSIS: The statistical analysis was performed using SPSS version 16 .0 software. The chi square test was used to determine the hypothesis of equality in the expression of the markers in relation to various histopathology parameter. The Pearson’s correlation coefficient was used to investigate the correlation between the parameters and positivity of Bcl2 and Glut1 . P<0 .05 was considered to indicate a statistically significant result. RESULTS: Bcl 2 and Glut 1 were sufficiently expressed in the OPMD and OSCC samples. Bcl 2 positivity showed insignificant difference with age, gender, site and histopathology grading. Glut 1 positivity showed significant difference with histopathological grading and was insignificant with age, gender, site. CONCLUSION: The expression of the Bcl2 and Glut1 show that there is some role in OSCC which may helps in assessing the disease process and modulating the treatment plan.

Item Type: Thesis (Masters)
Additional Information: 241921602
Uncontrolled Keywords: BCL 2, GLUT 1, Immunohistochemistry, Oral squamous cell carcinoma, Oral potential malignant disorder.
Subjects: DENTAL > Oral Pathology and Microbiology
Depositing User: Subramani R
Date Deposited: 08 May 2022 08:41
Last Modified: 14 May 2022 06:35
URI: http://repository-tnmgrmu.ac.in/id/eprint/19772

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