Rohini, S (2022) Association between Salivary Free Testosterone Levels and Serum Luteinizing Hormone, Follicular Stimulating Hormone, Testosterone Levels in Women with Polycystic Ovarian Syndrome: A Cross Sectional study. Masters thesis, Ragas Dental College and Hospital, Chennai.
Full text not available from this repository.Abstract
AIM OF THE STUDY: To determine the association between salivary free testosterone levels and serum Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH) and free testosterone levels amongst Polycystic ovarian syndrome (PCOS) patients. OBJECTIVES: 1. To select women with PCOS as defined by two of the three Rotterdam’s criteria16 between the age group of 18-35 years. 2.To estimate the serum Luteinizing Hormone, Follicle Stimulating Hormone and free testosterone levels. 3. To estimate the salivary free testosterone level. 4. To determine the association between salivary testosterone levels and serum Luteinizing Hormone, Follicle Stimulating Hormone and free testosterone levels. METHODOLOGY: This cross-sectional study was designed to determine the association between the salivary free testosterone levels with serum LH, FSH and testosterone, which was carried out among the PCOS women between the age group of 18-35 years visiting the Department of Obstetrics and Gynaecology, of two medical centres. Overall, 80 patients, out of which 35 patients from one medical centre and 45 patients from another medical centre who met the eligibility criteria were recruited for the study. Intra-oral examination was carried out with the help of artificial light source to assess the oral health status of the study subjects with a Mouth mirror and Explorer to check for the oral lesions. The saliva sample collection was done by passive drooling method as per the protocol described by Granger et al. (2007). Saliva samples were immediately frozen to -80°C and batch analyzed for free salivary testosterone using ELISA technique. Blood samples as part of their medical evaluation was drawn from the antecubital vein and collected in a centrifuge tube on the 2nd day of the menstrual cycle of the patient for the determination of luteinizing hormone, follicular stimulating hormone and free testosterone levels. Determination of serum luteinizing hormone and follicular stimulating hormone levels were done by Electrochemiluminescence immunoassay (ECLIA) method and serum free testosterone levels was determined by Radioimmunoassay (RIA) method. RESULTS: The mean salivary free testosterone was 37.43 ± 3.65 pg/ml and mean serum free testosterone was15.34 ± 6.63 pg/ml in the PCOS cases. This study showed a significant positive correlation between salivary free testosterone, measured by enzyme linked non isotopic immunoassay (ELISA), and serum free testosterone measured by radio immunoassay (RIA) (r=0.67, p=0.002). Also, this study demonstrated a strong correlation between salivary testosterone and LH/FSH ratio (r=0.83, p=0.000). CONCLUSION: This study concludes that there is an association between salivary free testosterone levels and serum Luteinizing Hormone, Follicle Stimulating Hormone, free testosterone levels. Hence, salivary testosterone is an attractive alternative for measuring free testosterone directly in the body. Since measurement of salivary free testosterone, offers great potential in facilitating epidemiological and biomedical research at the population level, further longitudinal studies are required to validate the use of salivary testosterone for the diagnosis and monitoring among PCOS patients.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 241923103 |
| Uncontrolled Keywords: | Polycystic ovarian syndrome, Luteinizing Hormone, Follicle Stimulating Hormone, Serum free Testosterone, Salivary Testosterone. |
| Subjects: | DENTAL > Public Health Dentistry |
| Depositing User: | Subramani R |
| Date Deposited: | 08 May 2022 05:45 |
| Last Modified: | 14 May 2022 08:09 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19762 |
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