Archana, V (2019) Cardioprotective Effect of Delonix Elata (L.) Leaf Extract against Doxorubicin Induced Myocardial Damage in Wistar Rats. Masters thesis, Institute of Pharmacology, Madras Medical College, Chennai.
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Abstract
The present study was chosen for the leaf of Delonix elata (L.) for the scientific evaluation of cardioprotective potential based on the presence of bioactive compounds which is used to prevent cardiotoxicity. Doxorubicin is an anthracycline antibiotic which is effectively used in the treatment of different malignancies. Nowadays its use is limited because of the drug induce cardiotoxicity in dose dependent manner. Several mechanisms are involved in DOX induced cardiotoxicity, but the major mechanism is oxidative stress. Antioxidant properties of several plants are experimentally proved and widely used as most effective agents against oxidative stress. ROS is the major causative factor for many diseases. Antioxidants are prevent the deleterious effect caused by these free radicals. In vitro antioxidant study and cardioprotective study was carried out in three extracts (petroleum ether, ethyl acetate and ethanol) of Delonix elata (L.) to choose effective extract for further in vivo studies. The antioxidant effect was evaluated by SOD assay and DPPH radical scavenging assay. The cardioprotective study was evaluated by using H9c2 (Rat Cardiomyocyte) cell line. Cell viability assay (MTT assay) was carried out. From this, the ethanolic extract of Delonix elata (L.) leaf possess potent antioxidant effect which exihibit their action at lower concentration. In cell viability assay the DOX treated cells showed decreased cell viability and increased cell death. The pretreatment with the plant extracts increased the cell viability and reduce the cell death. From this also the ethanolic extract of Delonix elata (L.) possess potent cardioprotective activity. So the ethanolic extract was chosen for further in vivo studies. Acute oral toxicity study was carried out for evaluating the safety and toxic effect of ethanolic extract of Delonix elata (L.) leaf. From this study the result showed the ethanolic extract of D.elata leaves extract does not have any toxic effect upto the dose of 2000mg/kg body weight. The In vivo study was conducted with 4 groups of wistar rats, 6 animals in each group. For assessing the cardioprotective activity, Doxorubicin induced cardiotoxicity model was used. The doses of plant extract was chosen for the test to be 200 and 400mg/kg body weight of the animal. In doxorubicin induced cardiotoxic rats showed significant decrease in body weight and looked very weaker when compared with the normal control group. The pre-treatment of EEDE leaf to Dox administered animals showed significant increase in body weight. The heart weight of Dox treated rats showed significant increase when compared with normal control group. The pretreatment of EEDE leaf to Dox administered animals showed significant decrease in heart weight. In Dox induced cardiotoxic group significantly decreased the haematological parameter values and the pretreatment of EEDE leaf to Dox administered animals shows that the haematological parameter values were increased. The elevation of cardiac marker enzyme level in serum was showed in Dox induced cardiotoxic group and it confirms the onset of myocardial injury. The pretreatment of EEDE leaf to Dox administered animals significantly decreased the cardiac marker enzyme level in serum. The antioxidant enzyme (SOD, CAT, GPx) level was decreased in Dox induced cardiotoxic rats heart homogenate which is compared with normal control group. The pretreatment of EEDE leaf to Dox administered animals showed significantly increased antioxidants level in heart homogenate. Dox induced cardiotoxic group increased lipid peroxidation parameter like MDA level and it was significantly decreased in EEDE treated group. Histopathological studies in isolated heart revealed that the pretreatment of EEDE leaf prevent the myocardial damage and restored the normal architecture of the heart. From this study, it is concluded that the ethanolic extract of Delonix elata (L.) was found to possess the cardioprotective activity. Further findings are needed to explore the possible mechanism of action of isolated compound from this plant against cardiotoxicity.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 261726051 |
| Uncontrolled Keywords: | Cardioprotective Effect, Delonix Elata (L.), Leaf Extract, Doxorubicin, Myocardial Damage, Wistar Rats |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ramakrishnan J |
| Date Deposited: | 02 May 2022 10:49 |
| Last Modified: | 02 May 2022 10:49 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19650 |
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