Nabi Shiba, V (2019) A New RP-HPLC Chromatographic Method for the Simultaneous Estimation of Doxylamine Succinate and Pyridaxone Hcl in Its Bulk and Pharmaceutical Dosage Form. Masters thesis, Jaya College of Paramedical Sciences, Thiruninravur, Chennai.
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Abstract
Methodology consists of the general principle in HPLC instrumentation protocol for method development and the detail about the proposed RP-HPLC method on the drug is included. Where the RPHPLC method in which determination of Doxylamine succinate and Pyridaxone HCl was carried out on a Symmetry C18 (4.6 x 150mm, 5μm, Make: Waters) using a mobile phase consisting of pH 3.5 phosphate buffer: Acetonitrile (30:70). The mobile phase was pumped at a rate of 1.0 ml/min and the detection was carried out at 254nm. The retention time of Doxylamine succinate and Pyridaxone HCl was found to be2.162 and 3.305 min respectively and linearity was in the range of 12-60μg / ml for Pyridoxine HCl and 20-100 μg / ml for Doxylamine succinate. The results obtained in newer RP-HPLC method for determination of Doxylamine succinate and Pyridaxone HCl are tabulated and also discussed about the developed RP-HPLC method. The proposed method is simple cost effective and gives reliable assay results with short analysis time (5min).The content of drugs in the formulation was found to be 60mg Ambroxol and 100mg Doxylamine succinate. The method was validated in terms of sensitivity, accuracy and precision and can be used for the routine determination of Doxylamine succinate and Pyridaxone HCl in pharmaceutical formulations. The above method does not suffer from any interference due to common excipients. Therefore the proposed RP-HPLC method could be successfully applied to estimate commercial pharmaceutical products containing Doxylamine succinate and Pyridaxone HCl.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 261731104 |
| Uncontrolled Keywords: | New RP-HPLC Chromatographic Method, Simultaneous Estimation, Doxylamine Succinate, Pyridaxone HCI, Pharmaceutical Dosage Form |
| Subjects: | PHARMACY > Pharmaceutical Analysis |
| Depositing User: | Ramakrishnan J |
| Date Deposited: | 29 Apr 2022 06:06 |
| Last Modified: | 29 Apr 2022 06:06 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19546 |
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