Latha, M (2020) Evaluation of Immunomodulatory Activity of Jasminum sambac (L.) Aiton Flowers by IN-VIVO Methods. Masters thesis, Nandha College of Pharmacy and Research Institute, Erode.
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Abstract
The use of potential natural medicines as immunomodulators is a new concept in medicine used as traditional medicine. An immunomodulator is a compound that can improve the body's defense mechanism. The importance of the body's defense system to health is to prevent or protect the body from infection and also the immune system into a physiological system to regulate body homeostasis. Herbs have been used extensively in ethnopharmacology as a potential immunomodulatory effect. The flowers of Jasminum sambac was collected on Dec 2019 from market in perundurai. It was then dried for one month and later powdered.This powder was then extracted with ethanol for 24 hrs using soxhlet apparatus. After extraction Phytochemical analysis was carried out to determine the presence of flavonoids,phenols, saponins, steroids, tannins etc., Ethanolic extract of Jasminum sambac flowers was dissolved in 1%cmc. Acute toxicity study was carried out and then selected different doses namely 200mg/kg and400mg/kg body weight. Immunostimulant activity is studied by using suppressing drug namely cyclophospamide and then by grouping animals into different groups. On day 0 all the animals was suppressed by cyclophospamide and then after day 1 to 14, all groups were treated with different doses. At the end of day 14 blood from all the groups were collected in EDTA tubes for estimation of complete blood count. In complete blood count following parameters are counted. White blood cells, Neutrophil , lymphocytes,monocytes, basophil, eosinophil, Red blood cell, platelet count , heamoglobin. For neutrophil adhesion test, On day 14 after administration of the extract, blood samples from rats in Group 1-5 were obtained by ventricular puncture and analyzed for total leucocyte counts (TLC) and differential leukocyte counts (DLC). After the initial counts, the blood samples were incubated with 80 mg/mL of nylon fibers for 15 min at 37 °C. The incubated blood samples were again analyzed for TLC and DLC. The product of TLC and % neutrophil were given as the neutrophil index (NI) of blood sample Percent neutrophil adhesion was calculated.These two test are done for innate immune response. For adaptive immune response antigen was prepared using sheep red blood cells. Heamagglutinating antibody titre test is carried out. On day 7 blood from second set of group of animals were collected and centrifuged to get serum. Antibody titre method was carried out to obtain hemagglutination antibody titer ( HA units/Μl). Delayed type hypersensitivity response was determined by inducing immunogenic response using SRBCs in rats with little modifications. After 7 days of immunization with SRBCs, the animals were administered 50 μl of SRBCs (0.5 x 109 /ml) into the right hind foot pad; while, the left hind foot pad was challenged with 50 μl of PBS. he thickness of right and left hind foot pads was measured by micrometer screw gauge or vernier calliper after 4 h, 8 h and 24 h of challenge. The difference in millimeter (mm) between right and left hind foot pad and percentage of footpad swelling was taken as DTH response to specific antigen. From all the study there was significant increase and this effect was due to phytochemical constituents such as tannins, steroids, saponins, phenols,flavonoids and aminoacids present in flowers.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 261825402 |
| Uncontrolled Keywords: | Immunomodulatory Activity, Jasminum sambac (L.) Aiton Flowers, IN-VIVO Methods |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ramakrishnan J |
| Date Deposited: | 11 Apr 2022 07:06 |
| Last Modified: | 11 Apr 2022 07:06 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19228 |
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