Dhanusu, N (2021) Method Development and Method Validation of Erythromycin with Isotretinoin by RP-HPLC. Masters thesis, J.K.K.Nattraja College of Pharmacy, Kumarapalayam.
Full text not available from this repository.Abstract
The empirical technique was developed by examining a variety of variables. First, the average absorbance the typical wavelength was 289 nm, and the purity to peak ratio was excellent. The injection volume was set at 20 μl, which resulted in a nice peak area. Inertsil C18, a solid peak shape chosen by ODS, was the column used for the analysis. The temperature in the room has been set to match the consistency of the medication solution. Because of the good peak area, the flow rate was chosen at 1.0ml/min, with acceptable retention duration and good resolution. Methanol: Acetonitral (70:30) was chosen as one of the mobile phase ratios with the best symmetrical peaks, and resolution. and for the planned investigation, this mobile strategy was adopted. The most recent recovery was determined to be linear and constant in the 98.0-100.25 range. Both the equipment and the technique have been proved to be accurate and within acceptable limits. The detection limits for Erythromycin and Isotretinoin were 0.707 and 0.096, respectively. The coefficient of correlation and curve fitting were discovered during the linearity study. Over the 20-80ppm target concentration range for both medicines, the analytical technique found linearity. The empirical has passed all sturdiness and robustness tests. On both occasions, the relative Standard deviation was within acceptable bounds. The developed HPLC method has the following advantages: • No tedious extraction procedures were involved. • The run time required for recording chromatogram was below 6.14 mins. • Suitable for the analysis of raw materials and formulations. Hence, the developed chromatographic method for Erythromycin and Isotretinoin. Is said to be rapid, simple, precise, accurate, and cost effective that can be effectively applied for the routine analysis in research institution, quality control Department in industries, approved testing laboratories, biopharmaceutical studies, and in clinical pharmacokinetic studies.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 261930201 |
| Uncontrolled Keywords: | Method Development, Method Validation, Erythromycin, Isotretinoin, RP-HPLC. |
| Subjects: | PHARMACY > Pharmaceutical Analysis |
| Depositing User: | Ramakrishnan J |
| Date Deposited: | 04 Apr 2022 05:20 |
| Last Modified: | 29 Aug 2022 04:26 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19148 |
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