Sudha Revathy, S (2015) Preclinical Evaluation of Safety and Therapeutic Efficacy of Siddha Herbo Mineral compound Kara Sooda Sathu Parpam for Kalladaippu Noi (Urolithiasis). Doctoral thesis, The Tamilnadu Dr. M.G.R. Medical University, Chennai.
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Abstract
The present study was carried to evaluate the safety and efficacy of the siddha herbo mineral drug, Kara sooda sathu parpam in the management of Kalladaippu noi (Urolithiasis) with animal experimentations and in vitro methods. The drug analysis was also carried out to explore the chemical nature of the drug. • The drug analysis revealed the presence of elements like calcium, sodium, boron, potassium, magnesium, zinc and iron. The groups present were carbonate, sulphate, chloride and nitrate. The XRD revealed the presence of calcium in oxide and carbonate form. The particle size ranged from nano to micro size which enhances the bioavailability of the drug. • In vitro anti-oxidant studies revealed that the drug is a good antioxidant and is efficacious as that of ascorbic acid both in DPPH and Nitric oxide scavenging assays. • The drug inhibited the growth of calcium oxalate crystals in vitro by single diffusion gel growth technique and the inhibition was dose dependent manner. • The drug exhibited a moderate anti-microbial activity against E.coli and Proteus mirabilis, which are the most common pathogens causing UTI on patients suffering from urolithiasis. • The acute and long term toxicity study revealed that the drug KSSP was safe up to 10 times the therapeutic dose as mentioned in the literature. The histopathological studies supported the same. • The anti urolithiatic activity by Ethylene glycol method revealed that the drug KSSP reduced the calcium and oxalate content in urine and kidney tissues. It was also found to have renal protective activity by reducing the renal markers which was increased in the calculi induced group. The results were confirmed by histopathological studies. The drug was efficacious both in preventive and curative aspects. The drug also increased the antioxidant catalase levels in kidney and liver tissues thus proving to be an anti-oxidant. • The anti urolithiatic activity by zinc implantation method revealed that the drug KSSP is effective both in preventive and curative aspects. The drug significantly reduced the stone size and X-ray findings too revealed the same. The dose 131.5 mg/kg was more effective than 87.5 mg/kg. • The diuretic study revealed that the drug KSSP increased the urine output and also the excretion of electrolytes. The activity was in par with the standard drug. • The drug also showed potent anti-inflammatory activity. To conclude the drug Kara sooda sathu parpam exerts its beneficial effect in Kalladaippu noi (Urolithiasis) by being a good anti urolithiatic, diuretic, antioxidant, anti-inflammatory and a moderate anti-microbial. RECOMMENDATIONS: The drug Kara sooda sathu parpam (KSSP) should be subjected to clinical trial to validate the efficacy of the drug for human use. • The drug is also recommended to be validated for its preventive effects and also as a supportive therapy to other conventional medical expulsive therapies. • The pharmacological activity by which the drug is helpful in the management of urolithiasis has been validated in this study. Hence further pharmacokinetic and pharmacodynamics studies will prove to useful in understanding the mechanism of action of the drug.
| Item Type: | Thesis (Doctoral) |
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| Uncontrolled Keywords: | Preclinical Evaluation, Safety, Therapeutic Efficacy, Siddha Herbo Mineral compound, Kara Sooda Sathu Parpam, Kalladaippu Noi, Urolithiasis. |
| Subjects: | AYUSH > Gunapadam |
| Depositing User: | Subramani R |
| Date Deposited: | 22 Jan 2022 05:38 |
| Last Modified: | 22 Jan 2022 05:38 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19051 |
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