Preclinical evaluation of effect of Karisalai Chooranam against restraint stress induced oxidative damage

Mary Princess Sulekha, S (2019) Preclinical evaluation of effect of Karisalai Chooranam against restraint stress induced oxidative damage. Doctoral thesis, The Tamilnadu Dr. M.G.R. Medical University, Chennai.


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Restraint stress is an established animal model for psychological stress. Restraint stress induces oxidative stress through elevation of corticosterone. Development of oxidative stress can be understood from the changes in the status of Antioxidant enzymes and of lipid peroxidation particularly in hypothalamus and hippocampus. Restraint stress induced oxidative stress disrupts functions of hypothalamus and hippocampus particularly disruptions in special recognition, memory and anxiety were observed. Moreover, Restraint stress induced oxidative stress altered the cytokine profile of animal, namely IL6, IL10 which are associated with psychological stress. KSC which is a herbal preparation from Siddha Medicine, consists of six medicinal plants, was standardised as per ministry of AYUSH, Government of India, standards. It showed its efficacy in preventing psychological stress induced abnormal deviations especially in spatial recognition memory functions and anxiety. The efficacy of KSC against detrimental effects of psychological stress, could be due its antioxidant potential. Possible mechanisms of action for its antistress activity are, by preventing development of oxidative stress induced disruptions in hypothalamus & hippocampus functions and by preventing development of dysregulation in cytokines which have important role in psychological wellbeing. From the present study, two research publications have already been made (Refer annexures) with respect to KSC. Further evaluation through clinical studies are required for human usage. IMPACT OF THE STUDY: Stress extracts a high price from our body as well as our mind. Many degenerative diseases are associated with free radical induced oxidative damage. There is great need for safe and effective prevention strategies to combat the ravages of stress on our body. Karisalai chooranam (KSC) preparation may be a promising alternative treatment on preventive aspect for a variety of degenerative diseases. In this study, KSC has been proved experimentally for its efficacy against restraint stress in an animal model. Hence, it can be used for its antistress and antioxidant potential. Moreover, the results of the standardization parameters from the present study can be used as a reference standard in future to find out batch to batch variations. This study acquires significance for its health care and standardization perspectives.

Item Type: Thesis (Doctoral)
Uncontrolled Keywords: Preclinical evaluation, Karisalai Chooranam, restraint stress, oxidative damage.
Subjects: AYUSH > Gunapadam
Depositing User: Subramani R
Date Deposited: 22 Jan 2022 01:45
Last Modified: 22 Jan 2022 01:45

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