Peddada Revathi, (2016) Evaluation of Cytotoxicity of Orthodontic Mini Implants in Two Different pH Concentrations: An In Vitro study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
AIM OF THE STUDY: The purpose of this in-vitro study was to evaluate the cytotoxicity of orthodontic mini implants in two different pH concentrations. MATERIAL AND METHODS: Three different companies of mini implants named 1. Dentos implants, Anchor Dentos Inc, Korea 2. Denticon, Dental Instrument Co, Mumbai, India and 3. SK Mini Implants, SK surgical, Om lab Services, Pune, India were selected and the cytotoxicity is evaluated at two different pH concentrations such as pH 4 and pH 7 at day 15, day 30 and day 60. Twelve implants of each company were analyzed for evaluation of their cytotoxicity by immersing them in a NaCl solution at pH 4 and pH 7. The eluates are collected after the day 15, day 30, and day 60. The cytotoxicity was evaluated on two different cell lines such as Human Osteogenic Sarcoma Cell Lines and Human Gingival Fibroblast cell lines by exposing them to the eluates collected at day 15, day 30 and day 60. The evaluation of cytotoxicity is done by means of MTT Assay. This cytotoxicity after the exposure of the cells to eluates is correlated with the amount of the metal ions released from the mini implants after day15, day 30 and day 60. The evaluation of the amount of metal ions released such as Aluminium, Titanium, Iron and Vanadium which are principle metal composition of the mini implants is assessed by Inductive Coupled Plasma Mass Spectroscopy (Agilent 7700, ICP-MS system Mass Hunter 4.1). The results were statistically analyzed with non parametric independent sample t test, one way ANOVA, Tukey’s HSD post hoc test for multiple comparisons. RESULTS: 1. When comparing the cell death at pH 4 and pH 7, cell death at pH 4 is always greater than the cell death at pH 7 in both U2OS and HGF cell in all the three companies of mini implants tested in the present study. 2. When comparing the cell death at three different time periods, such as day 15, day 30 and day 60, there is a gradual increase in the cell death from day 15 to day 30 and from day 30 to day 60 in all the mini implants tested. 3. When comparing the cytotoxicity at day 60 in both U2OS and HGF; Dentos revealed a highest cell death followed by SK Implant and least cell death was observed in Denticon mini implant. 4. When comparing the total amount of metal ion released at day 60, Dentos showed a highest amount of metal ion release followed by SK Implant and least amount of metal ion release was observed in Denticon implant. 5. Cytotoxicity at day 60, can be correlated to the ICP-MS values in day 60, that the amount of total metal ions leached was more in Dentos Implants followed by SK Implants and least was in Denticon Implants. CONCLUSIONS: From this study it is evident that mini implants with double Titanium oxide layer which thought to have high corrosion resistance might have higher cytotoxic effect when exposed for longer duration. This can be because of presence of high concentration of Aluminium and Venidium. However these elements are added in order to increase the strength, modulus of elasticity and formability of the implant. Thus, it is prudent to conclude that as far as the cytotoxic effects are concerned, the Denticon Implants are superior to the other implants used in our study. It is because Denticon Implant contains less concentration of Aluminium, Iron and also the total amount of metal ions leached was significantly less. However its strength, and fracture resistance during insertion should be validated in the further studies.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Cytotoxicity, Orthodontic Mini implants, pH concentration, Metal Ion Release. |
| Subjects: | DENTAL > Orthodontics and Dentofacial Orthopedics |
| Depositing User: | Subramani R |
| Date Deposited: | 28 Sep 2021 13:27 |
| Last Modified: | 28 Sep 2021 13:27 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/18143 |
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