Saranya, V (2016) Expression of Stage Specific Embryonic Antigen-4 (SSEA-4), in Dental Pulp Isolated from Human Permanent Teeth: An Immunocytochemical study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: Dental Pulp Cells (DPCs) are unique and viable source of adult mesenchymal stem cells. These cells have a capacity to differentiate into multiple lineage. AIM OF THE STUDY: To isolate and characterize mesenchymal stem cells from dental pulp in α-Modified Minimum essential medium (α-MEM) and to study the expression of Stage Specific Embryonic Antigen-4 (SSEA-4) - an embryonic marker in their 1st, 2nd and 3rd passages. MATERIALS AND METHODS: Isolation of pulp tissue was done from 30 permanent teeth samples. Among 30 samples, growth characteristics and morphology was assessed and immunocytochemistry was also done for SSEA-4 for two different samples in the 1st, 2nd and 3rd passages of the culture. RESULTS: 4 successful cultures yielded sufficient cells for characterization. Among 4, growth characteristics and morphology was assessed for 2 samples. There was gradual increase in the mitotic to post mitotic phenotype. The average Population Doubling Time (PDT) and seeding efficiency was 3.26 (days) and 70.15% respectively. Immunocyotochemical analysis was done using SSEA-4 which showed negative expression in the cells of all the passages. CONCLUSION: Dental Pulp Stem Cells (DPSCs) are the excellent source of stem cells. The stem cells were successfully isolated from dental pulp of 4 different third molars. The growth characteristics and morphology was assessed for two samples. SSEA-4 expression was not seen in all the 3 passages of the two samples. But the expression was seen in positive controls. We believe that cells of the later passages could turn out to be positive for SSEA-4, which would comprise of differentiated cells.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Stem cells, Stage Specific Embryonic Antigen-4 (SSEA-4), Immunocytochemistry. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 24 Sep 2021 16:40 |
| Last Modified: | 24 Sep 2021 16:40 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/18090 |
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