Nikhita Rebekah David, (2016) Evaluation of Expression of B7-H3 in Gingival Fibroblasts: A Cell Culture, Immunocytochemistry and Flowcytometry study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: Periodontal disease is a chronic inflammatory condition that leads to the breakdown of the periodontium, and ultimately the loss of the tooth. This breakdown of supporting tissues is by and large host mediated by inflammatory/immune cells including T cells. An important step in T cell activation is co stimulation. B7-H3 is a recent addition to the B7 family of co stimulatory molecules and is found to be present on the surface of many cells, including fibroblasts, raising the possibility of gingival fibroblasts being involved in immune modulation of periodontal disease. MATERIALS AND METHODS: Gingival tissue was collected from 7 patients who were referred to the Department of Periodontics for a crown lengthening procedure. Tissue samples were transferred to the lab and 5 samples were cultured successfully. Fibroblasts were characterized by CD40 and vimentin. Evaluation of B7-H3 was done by immunocytochemistry using the intensity of staining. The intensity of staining was determined by two examiners and the interexaminer variation was determined by kappa analysis. The results obtained for B7H3 by immunocytochemistry were confirmed by flowcytometry. RESULTS: The gingival fibroblasts showed a positive staining for the expression of B7-H3 (LI- 47.46) ranging from moderate to intense. Kappa analysis revealed good inter-examiner agreement (0.718). Flowcytometry analysis revealed that 99.3% of the gingival fibroblasts were shown to express B7-H3. CONCLUSION: Gingival fibroblasts express B7-H3 and thereby may have a secondary role in modulating immune functions in the periodontium. Further studies including periodontal disease models are required to identify their exact role.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | B7-H3, CD-276, Periodontitis, T cells, Co stimulation, Fibroblasts, Immunocytochemistry, Flowcytometry, Antigen presenting cells. |
| Subjects: | DENTAL > Periodontology |
| Depositing User: | Subramani R |
| Date Deposited: | 22 Sep 2021 06:58 |
| Last Modified: | 22 Sep 2021 06:58 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/18068 |
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