Microleakage Assessment of New Zinc Oxide Nano Particle Sealers, by Glucose Penetration Model: An In Vitro study

Sandeep Nuli, (2016) Microleakage Assessment of New Zinc Oxide Nano Particle Sealers, by Glucose Penetration Model: An In Vitro study. Masters thesis, Ragas Dental College and Hospital, Chennai.

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Abstract

AIM OF THE STUDY: Evaluation and comparison of the microleakage for Nano zinc oxide powder sealers, with the conventional zinc oxide eugenol sealer and AH Plus sealer using glucose penetration model. METHODOLOGY: 90 extracted human mandibular premolar teeth with intact root were taken and radiographed, teeth with single root canal were selected for the experiment. The teeth were decoronated horizontally below the level of the cementoenamel junction to equal lengths of 15mm. The samples were divided into 6 groups each containing 15 samples. 4 experimental groups and 2 control groups were taken for the study. All the samples were cleaned and shaped using K file and Gates Glidden drills by step back technique. The apical portion was enlarged upto #50 K file and the coronal flaring was done upto #6 Gates Glidden drill. The teeth were obturated using 2% gutta percha by cold lateral condensation. The samples in Group A used Nano Zinc oxide powder (30nm) sealer, Group B used Nano Zinc oxide powder (240nm) sealer, Group C conventional zinc oxide eugenol sealer, Group D AH plus sealer and in positive control group the teeth were obturated without using a sealer and negative control group samples were obturated using conventional eugenol sealer, the external root surfaces in the negative control group were completely covered with sticky wax including the coronal access opening and apical foramen. The obturated material in the experimental groups was condensed with a hand plugger leaving apical 4 mm of obturating material and was subjected to microleakage test. Glucose penetration model was used in the present study to evaluate the amount of microleakage. 1mmol/L glucose was used as tracer in the present study in 0.2% sodium azide solution. The glucose leaked was subjected to spectrophotometric analysis. The samples were observed for duration of 1 month. The readings were obtained on day 1, day 10, day 20 and day 30. RESULTS: The mean microleakage value of Group A did not show statistically significant difference with Group B on day 30 (p 0.983). The mean microleakage value of Group B did not show statistically significant difference with Group D (p 0.076). Group A and B had mean microleakage values statistically significant compared with Group C (p 0.000). CONCLUSION: Based on the present study it can be concluded that Nano zinc oxide powder (30nm) sealer showed lesser microleakage compared to Nano zinc oxide powder (240nm) sealer, Conventional zinc oxide sealer and AH plus sealer.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Micro leakage, Nano Zinc oxide powder sealer, Glucose penetration model.
Subjects: DENTAL > Conservative Dentistry and Endodontics
Depositing User: Subramani R
Date Deposited: 21 Sep 2021 03:20
Last Modified: 21 Sep 2021 03:20
URI: http://repository-tnmgrmu.ac.in/id/eprint/18055

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