Padmavathi, E (2011) A Study on Correlation of Clinical Features with Serodiagnosis and Molecular Charactrisation of Chikungunya. Masters thesis, Madras Medical College, Chennai.
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Abstract
INTRODUCTION : Chikungunya fever is an important arthropod-borne virus (arbo virus) Disease. Until recently it attracted only minor interest in the medical Community and did not evoke the fear associated with other arboviruses, Such as dengue and west nile viruses. The recent resurgence of chikugunya Fever has drawn global attention due to its explosive onset, rapid spread, high Morbidity and myriad clinical manifestations. Chikungunya virus (CHIK V) produces a dengue-like illness in humans, characterized by fever, rash, and severe arthralgia persisting for a few weeks to several months. The most significant characteristic of Chikungunya is the prolonged arthralgic syndrome that primarily affects the peripheral small joints associated with excruciating pain. The disease is generally non-fatal and the acute phase resolves within 3–4 days leaving the arthralgic syndrome persisting for some more time. The clinical course of the acute phase of infection has been well characterised during previous epidemics in African countries, the Indian subcontinent and Southeast Asia. It involves an incubation period lasting between two to six days, followed by the abrupt onset of fever associated with intense diffuse muscle and joint pain. Headache, photophobia, nausea, vomiting, diarrhea and a Maculopapular or morbiliform skin rash may accompany these symptoms. Treatment is mainly symptomatic, with remission observed in most patients Some days after the infection. This study was undertaken to study the incidence, clinical presentation and molecular characterisation of chikungunya virus in patients presenting with fever and arthralgia in Government General Hospital, Chennai. AIMS OF THE STUDY : 1. To study the incidence of chikungunya among patients with fever in Government General Hospital, Chennai. 2. To determine the seropositivity of chikungunya cases. 3. To determine the molecular characterisation of chikungunya virus. MATERIALS AND METHODS : Blood samples from 150 patients with clinical features suggestive of Chikungunya fever were included in this study. The samples were collected aseptically and serum was separated and stored at -70ºC. INCLUSION CRITERIA - The following patients were included in this study. Patients who had sudden onset of fever, rash, joint pain, chills, headache, nausea, vomiting, low back pain, conjunctival congestion and abdominal pain. All age groups . Both males and females. EXCLUSION CRITERIA - Patients with known history of chronic osteoarthritis, autoimmune diseases like rheumatoid arthritis, Systemic Lupus Erythematoses and any history of previous surgery of the joints were excluded. CONCLUSION : The incidence of chikungunya was 0.49% among patients attending the fever clinic and patients admitted in Government General Hospital, Chennai. The seropositivity of chikungunya cases was 41.33%. Dengue co-infection with chikungunya was 8%. Leptospiral co-infection with chikungunya was 9%. RT-PCR positivity among forty patients was 47.5%. Serum samples need to be collected at a very early period (1-3 days) within onset of fever to determine the serotype by RT-PCR. Serological diagnosis should be done in all clinically suspected Chikungunya cases to help in early initiation of treatment, thereby, minimizing the morbidity.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Correlation ; Clinical Features ; Serodiagnosis ; Molecular Charactrisation ; Chikungunya. |
| Subjects: | MEDICAL > Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 03 Aug 2017 01:18 |
| Last Modified: | 03 Aug 2017 01:18 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/1750 |
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