Isolation of Nonfermenting Gram Negative Bacilli from Intensive Care Unit Patients with Special Reference to Metallobeta Lactamase Production in Pseudomonas.

Mathavi, S (2008) Isolation of Nonfermenting Gram Negative Bacilli from Intensive Care Unit Patients with Special Reference to Metallobeta Lactamase Production in Pseudomonas. Masters thesis, Stanley Medical College, Chennai.

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Abstract

INTRODUCTION : The term “non-fermenters”(nfs) refers to a group of aerobic, nonspore forming Gram negative bacilli (GNB) that are either incapable of utilizing carbohydrate as a source of energy or degrade them via “oxidative” rather than fermentative pathways. In addition, they possess cytochrome oxidase activity and fail to grow or grow poorly on macconkey agar. Although frequently considered to be commensals or contaminants, the pathogenic potential of non-fermenters has been established beyond doubt by their recurrent isolation from clinical materials and their association with human disease. The critically ill patients receiving life saving therapy in Intensive Care Unit (ICU) often requires broad spectrum antibiotics, insertion of various indwelling devices, nutritional support and immuno-suppressive therapy. These factors along with underlying debility and immunosuppression account for the fact that though only 5-10% of all hospital beds are in ICU, these patients happen to be victims of over 20% of all hospital acquired infections. It is in this modern context of intensive treatment that the importance of nonfermenters as a nosocomial pathogen has increased. AIM AND OBJECTIVES : 1. To isolate non-fermentative Gram negative bacilli in various clinical samples processed from patients admitted in Intensive Care Unit in Government Stanley Hospital. 2. To establish the significance of their isolation. 3. To study the susceptibility pattern of these organisms to institute early and effective treatment. 4. To find out the emerging pattern of resistance in these organisms by phenotypic methods. CONCLUSION : Infections due to NFs pose a challenge to the ICU patients. NFGNB, normally a saprophyte, cause serious infections in immunocompromised and hospitalized patients especially those admitted to ICU. Of the various agents of NFs, Pseudomonas was the commonest organism to be isolated. Multidrug resistant Pseudomonas were observed. Amikacin was the highly effective antibiotic against most of the isolates. Increased incidence of MBL producing Pseudomonas had been observed. Mortality rate were high among MBL producers than non-producers.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Isolation ; Nonfermenting Gram Negative Bacilli ; Intensive Care Unit Patients ; Special Reference ; Metallobeta Lactamase Production ; Pseudomonas.
Subjects: MEDICAL > Microbiology
Depositing User: Subramani R
Date Deposited: 26 Jul 2017 05:55
Last Modified: 26 Jul 2017 05:55
URI: http://repository-tnmgrmu.ac.in/id/eprint/1722

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