The Study of Prevalence of Dengue Sero Type in Madurai by Molecular Methods.

Lallitha, S (2007) The Study of Prevalence of Dengue Sero Type in Madurai by Molecular Methods. Masters thesis, Madurai Medical College, Madurai.


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INTRODUCTION : The arthropod-borne or arboviruses are a group of infectious agent that are transmitted by blood sucking arthropods from one vetebrate host to another. Replication in the vertebrate host produces sufficient viremia that will infect other blood feeding arthropods. The vector acquires a Lifelong infection through ingestion of blood from a viremic vertebrate. The virus multiplies in the tissues of the arthropod without the evidence of disease or damage. Some arboviruses are maintained in nature by Transovarian transmission in arthopods. More than 500 viruses are now included in the international Catalogue of arboviruses. Any haemophagus arthropod can serve as a Vector of an arbrovirus. Recognised vectors include mosquitoes, ticks, Sand flies, swallow bugs and possibly mites. Except for urban yellow Fever, dengue and chikungunya virus, humans are typically dead end Hosts for the viruses. Generally lower vertebrates such as avians and Rodents serve as vertebrate hosts for a few of these viruses. The major Arboviral disease world wide are yellow fever, dengue, Japanese Encephalitis, St. Louis Encephalitis, Western Equine Encephalitis, Eastern Equine Encephalitis, Russian spring summer encephalitis, West Nile and Sand fly fever. AIM AND OBJECTIVE : To study the epidemiology of Dengue in Madurai by screening the suspected Dengue cases admitted at Government Rajaji Hospital, Madurai. To study the sero prevalence of Dengue infection in these cases by subjecting the serum samples to IgM / IgG spot ELISA and IgM capture ELISA. To isolate the Dengue virus from the IgM positive samples by inoculating into the suckling mice. To confirm the presence of Dengue antigen in the brain of suckling mice by haemagglutination (HA) technique. To confirm the presence of Dengue virus in the brain isolates by subjecting to RT-PCR. To find the type of the Dengue virus by single tube multiplex PCR by sero type specific amplicons. CONCLUSION : The study of prevalence of dengue sero type in Madurai by molecular methods revealed the following: Dengue cases were common during January 2006 – April 2006, due to the unusual rains in December 2005 and March 2006. More number of males in the age group 0 – 15 years were affected. The commonest symptom demonstrated was fever with myalgia and commonest sign was rashes. The spot IgG / IgM and IgM capture ELISA revealed that 12% of the affected were positive for IgM antibodies. Dengue virus antigen was prepared from the brain of the suckling mice by inoculating the IgM positive samples and the antigen titre was confirmed by HA technique taking into account 80 – 320 as the range of the titre value. These isolated viruses were subjected to RT-PCR and 40% of them showed amplification of 511 C-Pre M- region confirming the presence of dengue virus. The sero type of dengue virus was found out by single tube multiplex PCR analysis employing dengue sero type specific amplicons demonstrating 290 bp amplicons suggestive of dengue sero type 3.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Prevalence ; Dengue Sero Type ; Molecular Methods ; Madurai.
Subjects: MEDICAL > Microbiology
Depositing User: Subramani R
Date Deposited: 24 Jul 2017 04:36
Last Modified: 24 Jul 2017 04:36

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