Bacteroides Fragilis in Sepsis – Microbiological Studies.

Sugumari, C (2007) Bacteroides Fragilis in Sepsis – Microbiological Studies. Masters thesis, Madurai Medical College, Madurai.

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Abstract

INTRODUCTION : Bacteria, the oldest forms of life on earth, are remarkably diverse And exist in astounding number. Diseases caused by bacteria include Some of the most common infections in the world, as well as some of Human kinds most important scourges, past, present and probably future. At the same time, each of us is colonized by more bacterial cells than we Have human cells in our bodies. Generally, this is a peaceful even Productive (symbiotic) relationship, but occasionally even these well Tolerated residents of the human biosphere cause disease.53 Infections may be caused by Gram positive or Gram negative Bacilli or cocci 81, and these were the first recognized bacterial agents of Disease. In recent years, classification has been dominated by genotype Especially relying on conserved molecules like 16srrna. In the future, Taxonomy, understanding of pathogenesis and diagnostics will be Increasingly based on genotype. Thus the knowledge of taxonomy and Molecular biology must be broadened. AIM AND OBJECTIVES : To find out the prevalence of Bacteroides fragilis among patients with sepsis. To perform antimicrobial susceptibility testing of Bacteroides fragilis. To identify metronidazole resistant Bacteroides fragilis. To confirm the resistant pattern of Bacteroides fragilis genomically by short gene sequencing. To correlate with clinical conditions. To provide guidelines to clinicians in treating cases with sepsis. CONCLUSION : This study on Bacteroides Fragilis in Sepsis – Microbiological studies among patients with sepsis from General Surgical, Surgical Gastroenterology, Burns, Orthopaedics and Obstetrics & Gynaecology wards revealed. Bacteroides fragilis was isolated from 32 of 175 samples processed. Thus the prevalence was 18.3%. The prevalence was high among the post operative wound infections (8%) commonly in the males in the age group 46 – 55 years and in the females in the age group 26 – 35 years, from the pus samples. Of the 32 samples, antimicrobial susceptibility testing of Bacteroides fragilis revealed only one of the organism was resistant to metronidazole. Genomic sequence of metronidazole resistance in an International Laboratory revealed that the homology of the resistant strain showed a difference of 2.4% with the sensitive strain, thus confirming the resistant pattern. The patient with metronidazole resistant Bacteroides fragilis infection has improved well with the combination of clindamycin with other antimicrobials. The resistant organism was isolated from a post operative wound infection thus giving a warning signal to the clinicians on emerging metronidazole resistance on nosocomial anaerobic infections in this hospital. In view of the emerging infections, surgeons were informed about the inclusion of clindamycin in the management of post operative wound infection.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Bacteroides Fragilis ; Sepsis ; Microbiological Studies.
Subjects: MEDICAL > Microbiology
Depositing User: Subramani R
Date Deposited: 24 Jul 2017 04:29
Last Modified: 24 Jul 2017 04:29
URI: http://repository-tnmgrmu.ac.in/id/eprint/1668

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