Pre-operative diagnosis and prognostic evaluation of carcinoma breast: A comparative analysis of conventional FNAC and liquid based cytology

Nandhitha, N (2016) Pre-operative diagnosis and prognostic evaluation of carcinoma breast: A comparative analysis of conventional FNAC and liquid based cytology. Masters thesis, Tirunelveli Medical College, Tirunelveli.

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Abstract

Background-Fine needle aspiration cytology (FNAC) and Liquid based cytology(LBC) are minimally traumatic techniques used in the pre-operative diagnosis of lesions of breast. They can be used to determine ER,PR and Her-2/neu during the pre-operative period. Aim of study - To compare the cytomorphological features of conventional FNAC and Liquid Based Cytology and to evaluate the role of these techniques in the diagnosis as well as in the determination of prognostic and predictive factors. Materials and methods-30 cases of FNAC proven malignant lesions of breast were chosen and the cytomorphological features of conventional FNAC were compared with that of Liquid based cytology smears prepared using UPrep and Nanocyt technology. ER,PR and Her-2/neu was performed on conventional FNAC and LBC smears and the diagnostic and prognostic value of these techniques were evaluated. Results-The LBC was found to be better in terms of cellularity, clear background and monolayers while the cell architecture and the cytoplasmic and nuclear details were better preserved in conventional FNAC. Immunocytochemistry on FNAC was found to be very effective in predicting the response of the breast cancer to neoadjuvant chemo or hormone therapy and to determine prognosis. Immunocytochemistry on LBC was not found to be as effective as in conventional smears. The intensity of staining was found to be very poor. Conclusion- Conventional FNAC and LBC, though they have their own merits and demerits, they have comparable diagnostic accuracy and a combination of these methods have superior diagnostic value. Immunocytochemistry on conventional FNAC is an effective tool to evaluate the prognostic and predictive factors of breast during the preoperative period while immunocytochemistry on LBC is not as effective as in LBC.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Fine needle aspiration cytology ; Liquid based cytology ; Neoadjuvant chemotherapy.
Subjects: MEDICAL > Pathology
Depositing User: Laksham S
Date Deposited: 13 Jul 2017 11:11
Last Modified: 26 Nov 2018 17:22
URI: http://repository-tnmgrmu.ac.in/id/eprint/1662

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