Malarvizhi, K (2016) Study of Expression of Hypoxia Inducible Factor - 1 Alpha in the Risk Prediction of Oral Epithelial Dysplasia and in Oral Squamous Cell Carcinoma: An Immunohistochemistry study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: Oral squamous cell carcinoma (OSCC) is a multistep process which includes combined effects of individual genetic predisposition and exposure to environmental carcinogens. The rapidly proliferating tumor has insufficient vascularization which limits oxygen diffusion resulting in the development of hypoxic region. Hypoxic micro-environment of tumor cell is an important step in the progression of OSCC with involvement of hypoxia inducible factor 1 alpha (HIF-1 alpha). AIM AND OBJECTIVES: To study the expression of HIF-1 alpha in mild, moderate and severe epithelial dysplasia, OSCC and normal mucosa by immunohistochemistry (IHC). MATERIAL AND METHODS: Immunohistochemical detection of HIF-1 alpha was done using polyclonal antibody and Poly Excel HRP/DAB chromogen detection system on 60 samples, which included mild epithelial dysplasia (15 cases), moderate and severe dysplasia (15 cases), oral squamous cell carcinoma (OSCC-15 cases) and the expression was compared with that of normal mucosa (15 cases). The positive control used for HIF-1 alpha was human normal kidney. RESULTS: The pattern of HIF-1 alpha staining in all the cases (N=60) was cytoplasmic. All the cases of OSCC showed 100% positivity for HIF-1 alpha expression. 93.3% of mild epithelial dysplasia and moderate to severe dysplasia showed positive expression when compared to normal which was 80% positive. Tissue localization of HIF-1 alpha stain in the suprabasal, keratin and connective tissue increased from normal mucosa to increasing grades of epithelial dysplasia. All the cases of well differentiated and poorly differentiated OSCC expressed HIF-1 alpha. 66.6% and 33.3% of moderately differentiated carcinoma expressed mild and moderate intensity respectively. CONCLUSION: Compared to that of normal mucosa, HIF-1 alpha expression was increased with increasing grades of epithelial dysplasia and increasing grades of OSCC. This indicates that HIF-1 alpha expression was found to increase with the severity of the lesion. Hence HIF-1 alpha could be considered as one of the marker comparing the stages of dysplasia.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | HIF-1 alpha, hypoxic micro-environment, oral epithelial dysplasia. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 21 Sep 2021 01:56 |
| Last Modified: | 21 Sep 2021 01:56 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/16509 |
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