Divya, B (2016) Detection of Human Papilloma Virus 16 in the Whole Mouth Fluid of Oral Cancer, Cervical Cancer Patients and Normal Individuals. Masters thesis, Ragas Dental College and Hospital, Chennai.
|
Text
242602316divya.pdf Download (9MB) | Preview |
Abstract
BACKGROUND: The association of Human Papilloma Virus (HPV) in cervical and oropharyngeal cancer has been established. There is growing evidence that HPV contributes to oral cancer along with alcohol and tobacco use. In our study we investigated the presence of HPV 16 in the whole mouth fluid of oral cancer patients, cervical cancer patients and normal individuals. AIM OF THE STUDY: To quantify the expression of HPV 16 in the whole mouth fluid of oral cancer patients, cervical cancer patients and normal individuals by quantitative real time polymerase chain reaction (qPCR). MATERIALS AND METHODS: DNA was extracted from the whole mouth fluid of 20 patients with oral cancer, 10 patients with cervical cancer and 10 normal individuals. The DNA was extracted and quantified by quantitative real time polymerase chain reaction. RESULTS: Out of 40 samples, in 8 patients (40%) with oral cancer, 4 patients (40%) with cervical cancer and 7 (70%) normal individuals, HPV 16 was isolated in the whole mouth fluid. The mean HPV 16 viral load was highest in the whole mouth fluid of cervical cancer patients followed by oral cancer patients and normal individuals. CONCLUSION: HPV 16 was detected in the whole mouth fluid of a subset of oral cancer patients, cervical cancer patients and normal individuals in this present study. The presence and effect needs to be studied to establish the etiologic role of HPV 16 in the pathogenesis of a subset of oral cancers.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | HPV 16, oral cancer, cervical cancer, whole mouth fluid, PCR. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 21 Sep 2021 02:03 |
| Last Modified: | 21 Sep 2021 02:03 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/16262 |
Actions (login required)
 |
View Item |
