Himana Nishara, S (2021) Evaluation of Significance of Computerised Nuclear Morphometry in Benign and Malignant Breast Aspirates. Masters thesis, Stanley Medical College, Chennai.
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Abstract
AIM OF THE STUDY: To evaluate the significance of computerised nuclear morphometry in benign and malignant breast aspirates. METHODOLOGY: • Fine needle aspiration sample to be obtained from cases of breast masses. • Cytology used to categorise breast aspirates into benign and malignant. • Around 50 nuclei/ case to be analyzed using the Image J 1.44C morphometric software for image processing, and analysis (JAVA) developed by the National Institute of Health, USA. • Only Cases with confirmed histopathological diagnosis shall be included in the study. • The following nuclear features are to be analyzed: • Radius (computed by averaging the length of radial line segments from the center of the nuclear mass to each of the points of the nuclear border). • Nuclear area (the area within the outlined nuclear perimeter). • Perimeter (the distance around the nuclear border). • Diameter (diameter of the circle with the same area as the outlined nucleus.) • Compactness of the cell nuclei calculated using the formula: Perimeter2/area. RESULTS: The predominant age group in Fibroadenoma was 21 to 30 years and left breast was predominantly involved. The predominant age group in fibrocystic change was 41 to 50 years of age and left breast was predominantly involved. The predominant age group involved in proliferative breast diseases was 31 to 40 years of age and right breast was predominantly involved. The predominant age group in malignancy was 41 to 50 years of age and both the breasts were equally involved. The mean nuclear area showed an increase from fibroadenoma to fibrocystic change, fibrocystic change to proliferative breast diseases, proliferative breast diseases to malignancy. The mean nuclear perimeter showed an increase from fibroadenoma to fibrocystic change, fibrocystic change to proliferative breast diseases, proliferative breast diseases to malignancy. The mean nuclear diameter showed an increase from fibroadenoma to fibrocystic change, fibrocystic change to proliferative breast diseases, proliferative breast diseases to malignancy. The mean nuclear compactness showed no significant increase or decrease in between the groups. In differentiating fibroadenoma and fibrocystic change, mean nuclear area and mean nuclear diameter were found to be statistically significant (p value<0.05). In differentiating fibrocystic change and proliferative breast diseases, mean nuclear area and mean nuclear diameter were found to be statistically significant (p value<0.05). In differentiating proliferative breast diseases and malignancy, mean nuclear area and mean nuclear diameter were found to be statistically significant.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 201813052 |
| Uncontrolled Keywords: | Significance, Computerised Nuclear Morphometry, Benign and Malignant Breast Aspirates. |
| Subjects: | MEDICAL > Pathology |
| Depositing User: | Subramani R |
| Date Deposited: | 05 May 2021 16:00 |
| Last Modified: | 10 Jan 2022 02:08 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/15941 |
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