Biotechnological Production and Characterization of Sophorolipids from Candida bombicola MTCC 1910

Sivachidambaram, P (2008) Biotechnological Production and Characterization of Sophorolipids from Candida bombicola MTCC 1910. Masters thesis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore.


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Candida bombicola MTCC 1910 was able to produce a maximum yield 98.5 gL-1 of sophorolipid only. The sophorolipids obtained were purified and characterized by evaluation of surface tension and CMC. The sophorolipid mixture obtained is identified by LC-APCI-MS. The identified sophorolipids is 17-L-[(2’-O-β-glucopyranosyl- β-D- glucopyranosyl)-oxy]-9-octadecenoic acid. This sophorolipid is used as a source for preparing a novel compounds of useful functionality such as glucoselipid-acid, 17-L-[(b-glucopyranosyl)-oxy-octadec-(9)-enoic acid)], [w-1]-hydroxyfatty acid and (17-L-hydroxy-cis-9-octadecenoic acid), which are commercially not yet available and are difficult to prepare employing organic synthesis. This could be useful intermediate for polyester and macrocyclic lactone production. Besides their contribution as surface active agents, modification of sophorolipid opens a new field of special chemicals. In recent years, microorganisms have found their application not only in the production of variety of metabolic products but also in the biotransformation of several chemicals. The genetically engineered microorganisms are also being used for the commercial production of some non-microbial products such as insulin, interferon, human growth hormone and immunological products like viral vaccines. Many complex bioconversions that are achieved by microorganisms, cannot be achieved by normal chemical means and this sophorolipid was obtained using Candida bombicola MTCC 1910. Chemically synthesized surface active compounds are widely used in the pharmaceutical, cosmetic, petroleum and food industries. However, with the advantages of biodegradability and production from renewable resource substrates, biosurfactants may eventually replace their chemically synthesized counter parts. So far, biosurfactants have only a few specialized applications because they have been economically uncompetitive. However, the use of cheaper substrates, optimal growth and production conditions coupled with novel and efficient multistep downstream processing methods and the use of recombinant and mutant hyperproducing microbial strains can make sophorolipids production economically feasible. A judicial and effective combination of these strategies might, in the future, lead the way towards largescale profitable production of sophorolipids and makes sophorolipids highly sought biomolecules for present and future applications as fine specialty chemicals, biological control agents, and new generation molecules for pharmaceutical, cosmetic and health care industries.

Item Type: Thesis (Masters)
Additional Information: Reg no. 26063656
Uncontrolled Keywords: Biotechnological Production ; Characterization ; Sophorolipids ; Candida bombicola MTCC 1910.
Subjects: PHARMACY > Pharmaceutical Biotechnology
Depositing User: Ravindran C
Date Deposited: 12 Jul 2017 06:40
Last Modified: 15 May 2018 16:45

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