Thilakavathy, P (2013) Phenotypic and Genotypic Methods for detection of Biofilm producing Coagulase Negative Staphylococci in a Tertiary Care Hospital. Masters thesis, Madurai Medical College, Madurai.
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Abstract
INTRODUCTION : Gram positive bacteria are one of the most common isolates in the clinical microbiology laboratory. They are widespread in nature and can be recovered from the environment or as commensal inhabitants of the skin, mucous membranes and other body sites mostly in human and animals. The ubiquity of these gram positive bacteria in nature makes the interpretation of their recovery from patients specimens occasionally difficult unless clinical manifestations of an infectious disease process are apparent. Recovery of these organisms from specimens should always be correlated with the clinical condition of the patient before their role in an infectious process can be established. The present study was undertaken to detect the prevalence of biofilm producing and nonproducing CoNS which were isolated from various clinical materials in our laboratory by three different phenotypic methods such as Congo red agar (CRA) method, Tube method (TM), Tissue culture plate (TCP) method, PCR for detection of the ica gene and to find out the reliable method from above which can be recommended for routine detection of biofilm production in CoNS. AIMS AND OBJECTIVES : 1. To find out the prevalence of CoNS in various clinical samples collected from Govt. Rajaji Hospital (GRH), Madurai. 2. To isolate and characterise the CoNS among the clinical samples. 3. To identify the biofilm producing strains of CoNS in clinical isolates by various phenotypic methods. 4. The antimicrobial susceptibility pattern among the biofilm producing and non biofilm producing CoNS isolates. 5. To compare the various phenotypic methods in the identification of biofilm producing CoNS with PCR and to find out the most sensitive and economic method which is close to PCR that can be recommended for routine screening of Biofilm production in Microbiological laboratories. MATERIALS AND METHODS : The present study was conducted in Government Rajaji Hospital, Madurai, attached to Madurai Medical College. The study period was from June 2011 to May 2012. Ethical committee clearance from the institution was obtained before starting the study and informed written consent was received from the patients before collecting the specimens. A total of 456 clinical samples were collected from the patients admitted in various wards at Government Rajaji Hospital, Madurai. Pus, wound swab samples (from infected bone & joint prosthetic implants, surgical site infections), indwelling catheter samples, blood samples, urine samples were collected from 456 patients admitted at Govt. Rajaji Hospital during the study period. Inclusion Criteria: 1. All age groups and both sexes were included. 2. Patients admitted to various wards (ICU, CCU, IRCU, Orthopeadic, plastic surgery, medicine) with signs and symptom suggestive of impending infections such as infected implants, surgical site infection, urinary tract infection, septicemia, pyrexia of unknown origin were included in this study. RESULTS : Samples of pus, wound swab, blood and urine collected from 456 cases admitted at Govt. Rajaji Hospital, Madurai were included in this study. This study included both sexes of all age group. Out of 456 samples, 165 from wound swab, 103 from pus, 96 from blood and 92 from urine. Among the 456 samples, 424 showed growth and 32 samples showed no growth. Among the 424 isolates, 252 Gram positive cocci (GPC) in groups were Staphylococci species and 172 were Gram negative bacilli (GNB). CONCLUSION : Coagulase negative Staphylococci are responsible for nosocomial infections at GRH, Madurai and Staphylococcus epidermidis is the most commonly isolated CoNS species. • Resistance among CoNS are increasing especially among the patients with the indwelling medical devices due to Biofilm production. • As infection caused by Biofilm producing bacteria are difficult to treat, early diagnosis and management is necessary to reduce morbidity and mortality. • This study was focused on finding out of a simple, economic and more accessible method with high sensitivity and specificity to identify Biofilm production. • Various phenotypic methods were compared with PCR and found that Tissue culture plate method showed high sensitivity and specificity and it is closer to PCR.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Phenotypic and Genotypic Methods ; Biofilm ; Coagulase Negative Staphylococci ; Tertiary Care Hospital. |
| Subjects: | MEDICAL > Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 14 Aug 2017 17:17 |
| Last Modified: | 16 Aug 2017 00:23 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/1463 |
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