Sangeetha, R (2020) Correlation of spectroscopic changes in tissue, saliva and salivary DNA in oral potentially malignant disorders and oral cancer: An In vivo study. Masters thesis, Tamil Nadu Government Dental College and Hospital, Chennai.
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Abstract
AIM OF THE STUDY: To correlate the fluorescent spectroscopic characteristics of tissue and saliva in oral potentially malignant disorders (OPMD) and oral squamous cell carcinoma(OSCC) and spectroscopic characterisation of salivary DNA using ethidium bromide dye in oral potentially malignant disorder and oral squamous cell carcinoma. OBJECTIVES: 1. To assess the auto fluorescence spectrum of tissue in OPMD and OSCC. 2. To assess the auto fluorescence spectrum of saliva in OPMD and OSCC. 3. To assess the auto fluorescence spectrum of salivary DNA in OPMD and OSCC. 4. To compare and correlate the spectrum of tissue with saliva of OPMD and OSCC. MATERIALS AND METHODS: A total of 80 patients with average age of 20 to 60 in both the gender were selected and divided into 3 groups. Group A included clinically and histopathologically proven cases of oral cancer, Group B1–clinically and histopathologically diagnosed cases of leukoplakia and group B2 clinically diagnosed cases of oral submucous fibrosis and group C –healthy controls. The tissue fluorescence spectrum was analysed using handheld fibro-optic probe attached to fluorolog spectrophotometer at 280nm. Whole unstimulated saliva was collected and fluorescence spectrum was obtained using fluorolog spectrometer at 280nm and salivary DNA fluorescence spectrum obtained after adding ethidium bromide using fluorolog spectrophotometer at 480nm wavelength. RESULTS: The fluorescent spectrum of tissue, saliva and salivary DNA was recorded and subjected to one way ANOVA and multivariate linear discriminate analysis. It was observed from the ANOVA test that there was statistically significant difference among the groups. The discriminant analysis for the fluorescence emission spectrum of tissue shows predictive accuracy of 85% between control group and group A, 95% between control group and group B1 100% between the control group and group B2. The discriminant analysis of salivary fluorescent spectrum shows predictive accuracy of 95% between control group and group A, 75% between control and group B1 and 80% between control and group B2. The discriminant analysis of fluorescent emission of salivary DNA shows predictive accuracy of 90% between control and group A, 95% between control group and group B1 and 65% observed between control group and group B2. CONCLUSION: The results of the study revealed that oral potentially malignant disorders and oral carcinoma can be diagnosed using auto fluorescence spectroscopy. In this study statistically significant difference observed between OSCC and control group based on the protein fluorescence in tissue and saliva, DNA fluorescence in saliva. Both saliva and tissue are efficient in diagnosing oral carcinoma at early molecular changes using fluorescence spectroscopy.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Fluorescence spectroscopy, Saliva, DNA, Ethidium bromide. |
| Subjects: | DENTAL > Oral Medicine and Radiology |
| Depositing User: | Subramani R |
| Date Deposited: | 25 Feb 2021 02:38 |
| Last Modified: | 25 Feb 2021 16:25 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14512 |
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