Detection of p16ink4a in Oropharyngeal and Upper Respiratory tract Squamous cell Carcinoma

Johnraj Suresh, M (2016) Detection of p16ink4a in Oropharyngeal and Upper Respiratory tract Squamous cell Carcinoma. Masters thesis, Chengalpattu Medical College, Chennai.

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Abstract

BACKGROUND: The incidence of HPV associated HNSCC is increasing over the past 30 years. It is a growing public health concern. It has been reported that tissues of HPV associated HNSCCs over express p16INK4a . Therefore p16INK4a is used as a surrogate marker to detect HPV associated HNSCC. Immunohistochemical detection of p16INK4a is an easy and simple technique than molecular detection of HPVs. Hence we investigated the presence of p16INK4a in HNSCCs. OBJECTIVES: The objectives of our study are: 1.To study the association of p16INK4a expression with HNSCC, thus with the HPV. 2. To compare the p16INK4a expression in different subsites of the HNSCC. (3)To correlate the level of p16INK4a expression with different grades of HNSCC. MATERIALS AND METHODS: A total sample of 60 cases were analysed during the period of June 2014 to August 2015. We performed IHC detection in sections of formalin fixed paraffin embedded tissue of HNSCC cases and correlated the various patterns of p16INK4a positivity with respect to histopathological diagnosis. RESULTS: In the present study, 75% of the HNSCC cases were above 50 years of age. HNSCC was more common in males with male to female ratio of 6:1. 93.33% of HNSCC cases were positive for p16INK4a, of which the most common pattern was diffuse nuclear and cytoplasmic staining (53.33%). CONCLUSION: In the present study, increased number of HNSCC cases were seen over expressing p16INK4a (93.33%). Oropharynx was the most common site for p16INK4a positivity in HNSCC cases (94.44%). Among the oral cavity SCC cases, tongue was the most common site involved (64%). Of the HNSCC cases, most cases (53.33%) had diffuse pattern of p16INK4a over expression. However, DNA detection based studies are needed to validate the utility of IHC detection of p16INK4a as a surrogate marker for HPV associated HNSCC.

Item Type: Thesis (Masters)
Uncontrolled Keywords: HNSCC ; p16INK4a ; HPV ; Head and neck cancer ; Squamous cell carcinoma.
Subjects: MEDICAL > Pathology
Depositing User: Laksham S
Date Deposited: 11 Jul 2017 09:02
Last Modified: 13 Jun 2018 01:25
URI: http://repository-tnmgrmu.ac.in/id/eprint/1449

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