Estimation of Human Salivary Survivin in Oral Potentially Malignant Disorders and in Oral Squamous Cell Carcinoma: A Diagnostic Aid

Pritha, R (2020) Estimation of Human Salivary Survivin in Oral Potentially Malignant Disorders and in Oral Squamous Cell Carcinoma: A Diagnostic Aid. Masters thesis, Madha Dental College and Hospital, Chennai.


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INTRODUCTION: Oral cancer is a major public health problem in the Indian subcontinent. Despite advances in the treatment modalities, the 5-year survival rate of oral cancer is still below 50% in most of the countries. This is because most of the patients are diagnosed in advanced stages and approximately two thirds die as a result of their disease. Hence, there is an urgent need in developing sensitive and specific tumour markers which can aid in the early diagnosis of oral cancer and also in detecting malignant transformation at an early stage in OPMD’s like OL, OSMF. This will ultimately improve the survival rate of OSCC. Survivin, a member of inhibitor apoptosis family plays a key role in both inhibition of apoptosis and cell division. It is increasingly expressed in OSCC and OPMD’s. Its degree of expression is directly related to aggressive phenotype and poor prognosis; however, it is present in a very low or undetectable amount in healthy adult tissues. Due to these features, survivin detection could be employed in OSCC diagnosis. In the recent era growing interest towards salivary markers led to the search for potential tumour marker in saliva that could provide diagnostic and prognostic information in OSCC. MATERIALS AND METHODS: The study material comprised of 15 saliva samples from each of the following groups such as OSCC, OL, OSMF& normal mucosa. Salivary survivin level were estimated using Enzyme Linked Immunosorbant Assay. The data analysis was accomplished using SPSS (IBM SPSS Statistics for Windows, Version 25.0, Armonk, NY: IBM Corp. Released 2017). Significance level is fixed as 5% (α = 0.05). To compare mean values between groups (OSCC, OL&OSMF) one-way ANOVA is applied followed by Tamhane’s post hoc tests was performed for multiple pairwise comparisons. RESULTS: The results of the study indicated a statistically significant increase in the salivary survivin level among the OSCC, OSMF &OL group when compared with the control group. When a pairwise comparison was done between OSMF with OSCC and control, statistically significant difference was observed, subsequently while comparing leukoplakia with control and also on comparing OSCC with leukoplakia, similar results were obtained. CONCLUSION: The present study demonstrated higher salivary survivin levels in OSCC patients when compared to healthy individuals and other OPMD’S groups. This suggests that Survivin can be used as an adjunctive diagnostic marker for OSCC detection. The study also shows significant increase in the level of survivin in OPMD’S than control group. Further, survivin can also be used as a better therapeutic target due to its selective over expression in most of the cancers. This indicates that Survivin molecular therapy along with conventional therapy could be a novel therapeutic strategy against oral cancer.

Item Type: Thesis (Masters)
Additional Information: 241721501
Uncontrolled Keywords: Oral Squamous cell carcinoma, Oral Leukoplakia, Oral Submucous Fibrosis, ELISA, Survivin.
Subjects: DENTAL > Oral Pathology and Microbiology
Depositing User: Subramani R
Date Deposited: 24 Feb 2021 17:02
Last Modified: 24 Feb 2021 17:02

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