Porkodi Sudha, J (2020) Assessment of Cytomorphometry of Human Buccal Cells in Gestational Diabetes Mellitus patients: A Cross Sectional study. Masters thesis, Vivekanandha Dental College for Women, Tiruchengode.
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Abstract
AIM OF THE STUDY: The aim of the study was to assess the cytomorphometric changes in the exfoliated buccal mucosal cells in Gestational diabetes mellitus patients. OBJECTIVES: The objectives of the study were, 1. To assess the cytomorphometry of exfoliated buccal cell in GDM patients during pregnancy. 2. To assess the cytomorphometry of exfoliated buccal cell in healthy pregnant women during pregnancy. 3. To compare the cytomorphometry of exfoliated buccal cells between healthy pregnant women and GDM patients. MATERIALS AND METHODS: This study consisted of a total sample size of 80 pregnant women. The participants were divided in to 2 groups which include 40 cases of GDM patients as group I and 40 cases of healthy pregnant women as group II. The smear was collected from the buccal mucosa of both the group using cyto-brush and stained with Rapid PAP staining method. The stained slides were viewed and the images of 100 cells from each slide were captured. By using image pro premier 9.1 software, Nuclear area (NA), Cytoplasm area (CA), Nuclear cytoplasmic ratio (N/C ratio) was measured from each captured image of cell. Statistical analysis was done using unpaired-T test for comparing the study group and control group. RESULTS: Result showed that the mean nuclear area in GDM group was 35±5.04 and in control group was 22.53±5.31. The mean cytoplasm area in GDM group was 1182.95±175.12 and in control group was 1109.60±155.75. The mean nuclear cytoplasmic ratio (N/C ratio) in GDM group was 38.04±8.02 and in control group was 57.85±9.83. Morphologic variations like binucleation, micronuclei, perinuclear halo in buccal cells obtained from GDM patients. There was a statistically significant increase in NA in GDM group than control group (p= 0.000), N/C ratio was markedly higher in control group than GDM group (p= 0.000). The CA did not exhibit a statistically significant difference between the two group (p= 0.051). CONCLUSION: Oral exfoliative cytology can be used as a simple chair side investigation that is quick, simple, less technically challenging, painless and non-invasive procedure for microscopic investigation of the mucosa in GDM patients. The results observed in this study might contribute to the general understanding of the alterations in the cellular pattern of oral mucosa cells in GDM patients, and provide health professionals with adjuvant diagnostic tool for the early diagnosis of GDM.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 241721451 |
| Uncontrolled Keywords: | Cytomorphomerty, Exfoliative cytology, Gestational Diabetes Mellitus, Pregnancy. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 24 Feb 2021 16:45 |
| Last Modified: | 26 Feb 2021 01:37 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14485 |
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